摘要
目的探讨核糖核酸腺苷脱氨酶1（ADAR1）在原发性肝癌中的表达及其与肝癌临床病理特征的关系。方法免疫组织化学及Western blot法检测ADARl在癌旁组织及肝癌肿瘤组织中表达；收集患者年龄、性别、肿瘤直径、门脉癌栓、肝背膜受侵、分化程度、血清甲胎蛋白（AFP）及细胞核增殖抗原（Ki-67）的表达，了解ADAR1与肝癌临床病理特征的关系。结果免疫组织化学检测结果显示，ADAR1在癌旁组织中ADAR1表达率为29．32％，而在肝癌肿瘤组织中表达率为64．50％，肿瘤组织ADAR1阳性率远高于癌旁组织，差异有统计学意义（P〈0．05）；Western blot结果表明，癌组织ADAR1表达量明显高于癌旁组织，差异有统计学意义（P〈0．05）；肝癌组织中的ADAR1表达水平与患者年龄、性别、肿瘤直径、门脉癌栓及肝背膜受侵差异无统计学意义（P〉0．05），而与分化程度、血清AFP及Ki-67的表达密切相关，ADAR1表达水平明显升高（P〈0．05）。结论ADARl在肝癌中表达增加，且与肝癌分化程度和血管生成基因相关，提示ADARl可能通过诱导肝癌分化及血管生成而促进肝癌发生侵袭转移。
Objective To investigate adenosine deaminase acting on RNA 1 （ ADAR1 ） in primary liver cancer （PLC） and its relationship with clinical pathology. Methods ADAR1 expression was detected by immunohistochemistry and Western blotting assay in cancer tissues and tumor adjacent tissue; age, gender, tumor size, portal vein tumor thrombus, liver back film invasion, differentiation, serum alpha fetal protein （AFP） and proliferation cell nuclear antigen （Ki -67 ） expression were collected to explore relationship of ADAR1 and clinical pathology. Results Immunohistochemical detection showed that ADAR1 expression rate of tumor adjacent tissue was 29. 3%, and tumor tissue was 64. 50%, there was significant difference of ADARI positive rate between tumor tissue and adjacent tissue （ P 〈 0. 05 ） ; Western blotting assay results showed that ADAR1 expression in cancer tissue was significantly higher than that in the tumor adjacent tissue, the difference was statistically significant （ P 〈 0. 05 ） ; There were no statistical significance between ADAR1 expression levels and patient age, gender, tumor size, portal vein tumor thrombus and liver back film invasion showed （ P 〉 0.05 ） , and the degree of differentiation, serum AFP and Ki - 67 expression is closely related to that ADAR1 expression level increased significantly （ P 〈 0.05 ）. Conclusion ADARI expression in PLC increased associated with tumor differentiation and angiogenesis gene, suggesting that ADAR1 may induce degree of differentiation and angiogenesis to promote liver cancer invasion.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2016年第7期1830-1832,共3页
Chinese Journal of Experimental Surgery
基金
河南省科技厅基础与前沿技术项目（112300410037）
河南省重点科技攻关计划项目（132102310385）
郑州市重点实验室项目（2015-9-23）
河南省医学科技攻关计划重点项目（201502018）
关键词
原发性肝癌
核糖核酸腺苷脱氨酶1
侵袭
转移
Primary liver cancer
Adenosine deaminasc acting on RNA 1
Invasion
Metastasis
