摘要
目的比较两种巢氏PCR方法在检测二期梅毒患者血液标本中的价值。方法针对tpp47和pol A,分别建立两个巢氏PCR扩增全血中的梅毒螺旋体DNA。结果在353例梅毒阳性全血中,n PCR-tpp47和n PCR-pol A分别检出142例(40.2%)和132例(37.4%),结果差异有统计学意义(χ2=5.79,P<0.05);两种巢氏PCR在血清学试验高滴度(RPR≥1:8或TPPA≥1:2 560)组的检出率明显高于低滴度组(RPR<1:8或TPPA<1:2 560)。结论在检测二期梅毒全血标本Tp DNA时,n PCR-tpp47优于n PCR-pol A;巢氏PCR方法可作为梅毒血清学试验的补充试验。
Objective To compare the value of two nested-PCR methods in detecting Treponema pallidum（Tp） isolates in whole blood from patients with secondary syphilis.Methods Two nest-PCR methods targeted at Tp-tpp47 and Tp-pol A were developed and used to amplify Treponema pallidum DNA in whole blood.Results Among 353 Tp seropositive whole blood samples,142（40.2%） and 132（37.4%） samples were respectively positive for n PCR-tpp47 and n PCR-pol A,indicating a statistically significant difference（P〈0.05）.The positive rates detected by the two nested-PCR methods in the group with high titers（RPR ≥ 1：8 or TPPA ≥ 1：2,560） were significantly higher than those in the group with low titers（RPR 1：8 or TPPA 1：2,560）.Conclusions The n PCR-tpp47 is superior to the n PCR-pol A in the detection of Treponema pallidum DNA in whole blood from patients with secondary syphilis,and it is a useful supplemental serological test for the diagnosis of syphilis.
出处
《实用预防医学》
CAS
2016年第8期904-907,共4页
Practical Preventive Medicine
基金
湖南省科技厅一般项目(2014TT2025)
