摘要
目的研究缺氧对肺腺癌A549细胞中micro RNA(mi RNA)表达谱的影响,分析靶基因参与的生物学功能和通路。方法基于mi RNA芯片技术和生物信息学分析方法,分析缺氧条件和正常氧条件下培养的A549细胞中差异表达的mi RNA,并预测该mi RNA的靶基因,分析靶基因参与的生物学功能和通路。结果本研究共筛选出14个差异表达mi RNA,包括9个在缺氧细胞中上调mi RNA和5个下调mi RNA。上调和下调mi RNA的靶基因都参与DNA转录、核染色质修饰等功能,并且都参与Wnt信号、转化生长因子-β信号和丝裂原活化蛋白激酶信号通路。结论缺氧的肺腺癌A549细胞中mi RNA表达谱发生显著改变,一些差异表达mi RNA对某些基因具有调控作用。
Objective To analyze the effect of hypoxia on mi RNA expression in lung adenocarcinoma A549 cells. Methods Based on mi RNA chip technology and bioinformatic analysis methods, differential expression of mi RNAs between hypoxia-treated A549 cells and normoxia-treated A549 cells were identified, and their target genes were predicted. Besides, biological functions and pathways of target genes were analyzed.Results In total, 14 differentially-expressed mi RNAs were screened, including 9 upregulated mi RNAs and 5downregulated mi RNAs in the hypoxia-treated A549 cells. Target genes of both upregulated and downregulated mi RNAs were enriched in GO terms, such as DNA transcription, chromatin modification, as well as a set of pathways, such as Wnt signaling, TGF-beta signaling and MAPK signaling. Conclusions In the hypoxic A549 cells, mi RNA expressions have significantly changed. A series of differentially-expressed mi RNAs regulate certain genes.
出处
《中国现代医学杂志》
CAS
北大核心
2016年第13期37-42,共6页
China Journal of Modern Medicine
基金
黑龙江省自然科学然基金(No:D201230)
