摘要
目的:探讨四君子汤含药脑脊液对体外培养的溃疡性结肠炎肠黏膜淋巴细胞功能的影响。方法:制备大鼠四君子汤和美沙拉嗪含药脑脊液,收集并分离12例健康体检者(作为正常对照组)和18例溃疡性结肠炎肠黏膜固有层淋巴细胞,分为5组:病理组、IL-12诱导组、空白脑脊液组、含药脑脊液组、美沙拉嗪脑脊液组;通过不同处理方式体外作用细胞后,采用CCK-8检测各组细胞增殖能力;流式细胞术检测其周期分布及CD4^+T细胞比例;ELISA检测细胞分泌IL-1、IL-6、TNF-ɑ细胞因子的能力;Western blot检测细胞内IL-2蛋白的表达。结果:与IL-12诱导组和空白脑脊液组比较,含药脑脊液组细胞增殖能力下降,细胞周期阻滞在G0/G1期,CD4^+T细胞比例明显减少,IL-1、IL-6、TNF-ɑ细胞因子分泌能力明显下降,IL-2蛋白表达水平显著下调。结论:四君子汤含药脑脊液在体外能降低溃疡性结肠炎肠黏膜淋巴细胞的功能,其机制可能与四君子汤调控其分泌IL-1、IL-6、TNF-ɑ等细胞因子相关。
Objective：To explore the effects of cerebrospinal fluid contained Sijunzi Decoction on function of colon mucosa lymphocytes of ulcerative colitis in vitro. Methods： Prepared the rat cerebrospinal fluid contained Sijunzi Decoction or Mesalamine. Collected colon mucosa of the 12 healthy （ as normal group ） and 18 ulcerative colitis, then isolated and obtained lymphocytes from these samples. The lymphocytes were divided into pathological group, IL-12 group, cerebrospinal fluid group cerebrospinal fluid contained Sijunzi Decoction group, cerebrospinal fluid contained Mesalamine group. After exposing to various treatment,the proliferative capacity was measured by CCK-8,the cell cycle and the ratio of CD4＋T was analyzed by flow cytometry （FCM）, the content of IL-1, IL-6, TNF-α was detected by ELISA. The expression of IL-2 were detected by Western blot analysis. Results：Compared the cerebrospinal fluid contained Sijunzi Decoction group with the IL-12 group or cerebrospinal fluid group,the proliferative capacity and the percentage of CD4＋T was decreased, the cells significantly arrested at G0/G1 phase, the secretory capability of IL-1, IL-6, TNF-α were obviously reduced; the expression of IL-2 was also significantly down-regulated. Conclusion：The cerebrospinal fluid contained Sijunzi Decoction could decrease the function of colon mucosa lymphocytes of ulcerative colitis,the mechanism involved in may be Sijunzi Decoction regulating the secretory capability of IL-1,IL-6 and TNF-α.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2016年第6期815-819,共5页
Chinese Journal of Immunology
基金
重庆市卫生与计划生育委员会中医药科技项目
关键词
四君子汤
含药脑脊液
溃疡性结肠炎
机制
Sijunzi Decoction
Cerebrospinal fluid contained drug
Ulcerative colitis
Mechanism
