摘要
对超声波辅助提取远志多糖的工艺进行优化,结果表明,超声波辅助提取远志多糖的优化工艺条件为:超声提取时间30min,超声提取温度67℃,液固比101(mL/g),在该条件下远志多糖的得率为(5.87±0.21)%(n=3);红外光谱显示远志多糖具有典型的多糖特征吸收峰,推测为不含糖醛酸的中性多糖;远志多糖具有一定的清除DPPH自由基(DPPH·)和羟基自由基(·OH)的能力,其对DPPH·清除能力IC_(50)值为0.83mg/mL,对·OH清除能力IC50值为1.29mg/mL。
The optimum condition of ultrasonic extraction of Polygala tenuifolia Willd.Polysaccharide（PTP）was investigated.The results showed,the optimum condition was ultrasonic extraction time30 min,ultrasonic extraction,temperature 67 ℃,ratio of water to raw material 101（mL/g）,and extraction 2times,the yield of polysaccharide was（5.87±0.21）%（n=3）.IR spectra predicted PTP had typical characteristic absorption peaks which were neutral polysaccharide and free from urinic acid.In vitro antioxidant activities showed PTP had effects on DPPH radical and hydroxyl radical.The IC50 of it against DPPH radical and hydroxyl radical was0.83mg/mL,1.29mg/mL.
出处
《食品与机械》
CSCD
北大核心
2016年第5期152-156,193,共6页
Food and Machinery
基金
河北省中医药管理局项目(编号:2015082)
河北中医学院博士启动基金(编号:BSZ2015005)
关键词
远志
多糖
超声波
红外光谱
抗氧化活性
Polygala tenuifolia Willd.
polysaccharide
ultrasonic
IR spectra
antioxidant activity
