摘要
目的:基于射精连续统一体理论,探讨该法筛选原发性早泄动物模型的可行性及实用性。方法:应用雄性SD大鼠49只和雌性SD大鼠32只,雌鼠去势后在实验前48 h和4 h皮下分别注射苯甲酸雌二醇20μg和黄体酮500μg诱导雌鼠发情,雄鼠与发情期雌鼠进行6次交配实验,每周1次。观察和记录雄鼠后3次的交配行为,包括骑跨潜伏期(ML),插入潜伏期(IL),射精潜伏期(EL),射精后间隔期(PEI)、骑跨次数(MF)、插入次数(IF)、插入比例(IR)和射精次数(EF)。根据EF平均值,将EF<1的雄鼠划分为"射精延迟",1.5<EF<2.5的雄鼠划分为"射精正常",而将EF>3的雄鼠划分为"射精过快"。结果:最终22只雄鼠纳入本研究,其中,"射精过快"雄鼠的EL值显著缩短,与其他两组相比,都具有明显的统计学差异。与此同时,IF值在"射精过快"组雄鼠表现为最低。3组雄鼠的ML时间无明显差异。结论:根据平均EF值可简单、方便地筛选出"射精过快"的雄鼠,该模型对深入研究原发性早泄有十分重要的意义。
Objective： To investigate the feasibility and practicability of estabhshing an animal model of primary premature ejaculation using the ejaculation distribution theory. Methods： We induced behavioral estrus in 32 ovariectomized female SD rats by subcutaneous injection of 20μg estradiol benzoate at 48 hours and 500 μg progesterone at 4 hours before mating them with 49 male rats once a week for six times. During the last three copulations, we observed the male animals for mounting latency （ML）, intromission latency （ IL）, ejaculation latency （ EL）, postejaculation interval （ PEI）, mounting frequency （ MF）, intromission frequency （ IF）, in- tromission rate （ IR）, and ejaculation frequency （EF）. Results ： Finally, 22 of the male rats were included in this study. The mean EF 〉3 was deemed rapid ejaculation, EF 〈 1 sluggish ejaculation, and EF 1.5 -2.5 normal ejaculation. The EL was significantly shorter in the rapid ejaculation group than in the sluggish and normal ejaculation groups. The IF was the lowest in those with rapid ejaculation. No statistically significant differences were observed in the ML among the three groups of rats. Conclusion ： Based on the mean ejaculation frequency, the male rats with rapid ejaculation were easily screened, and this animal model may play an important role in exploring the mechanisms of primary premature ejaculation.
出处
《中华男科学杂志》
CAS
CSCD
北大核心
2016年第7期579-583,共5页
National Journal of Andrology
基金
国家自然科学基金(81501245)~~
关键词
性行为
射精
原发性早泄
动物模型
大鼠
sexual behavior
ejaculation
primary premature ejaculation
animal model
rat
