摘要
目的分析PTEN和LKB1在非小细胞肺癌中的表达特点以及二者的相关性。方法收集74例非小细胞肺癌患者蜡块,以免疫组织化学法检测PTEN及LKB1的表达;通过分析临床相关指标与PTEN及LKB1表达的相关性,探讨PTEN及LKB1表达的临床意义。结果 PTEN和LKB1的表达阳性率分别为47.3%和70.3%,LKB1表达阴性样本多伴有PTEN阴性表达,统计学分析发现LKB1与PTEN表达呈正相关(r=0.287,P=0.017);PTEN和LKB1在非小细胞肺癌中的表达与性别、年龄、病理类型无关(P均>0.05),PTEN表达缺失与淋巴结转移(P=0.017)、侵袭(P=0.008)及临床分期(P=0.019)相关,LKB1的表达缺失与肺癌侵袭(P=0.043)和临床分期(P=0.003)相关;联合PTEN和LKB1表达情况分析,同时为阳性表达的肺癌病例出现淋巴结转移和/或侵袭的比例低于同时为阴性表达者。结论 PTEN和LKB1的表达与非小细胞肺癌的发生及恶性进展有关,且可能存在协同作用;检测两个蛋白表达可作为非小细胞肺癌预后的判断依据。
Objective To analyze the correlation between the expression of PTEN and LKB1 in non-small cell lung cancer（NSCLC）.Methods Paraffin blocks of 74 cases of NSCLC patients were collected,and the expression of LKB1 and PTEN were detected by immunohistochemistry.The clinical related index and the correlation with the expression of PTEN and LKB1 were analyzed to explore the expression clinical significance of them.Results The PTEN and LKB1 expression positive rate were 47.3% and 70.3%,respectively.LKB1 expression negative samples were mostly associated with PTEN negative expression.Statistical analysis showed that LKB1 was positively correlated with PTEN expression（r=0.287,P=0.017）.The expression of PTEN and LKB1 in NSCLC had no relationship with gender,age and pathology type（P〉0.05）.The expression of PTEN is closely associated with lymph node metastasis（LNM,P=0.017）,invasion（P=0.008）and clinical stage（P=0.019）.And lack expression of LKB1 was closely associated with tissue invasion（P=0.043）and clinical stage（P=0.003）.Patients with PTEN and LKB1 positive showed more LNM and/or invasion than those with them negative.Conclusion The expression of PTEN and LKB1 may be related to the occurrence and progression of NSCLC,and may have a synergistic effect,and it can be used as a judgment of the prognosis of NSCLC.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2016年第4期507-511,共5页
Journal of Sichuan University(Medical Sciences)
基金
云南省科技厅-昆明医科大学应用基础研究联合专项资金项目(No.2012FB093)资助
