摘要
目的:通过观察丙泊酚麻醉对老年大鼠学习记忆影响以及海马CA1区NMDAR2B表达的变化,探讨丙泊酚对术后认知功能障碍(postoperative cognitive dysfunction,POCD)的影响。方法:雄性SD老年大鼠随机分为对照组、丙泊酚组,每组20只。丙泊酚组给予50 mg·kg-1丙泊酚腹腔麻醉,对照组给予相同剂量的乳剂,麻醉后1 d进行Morris水迷宫测试,结束后采集大鼠海马,采用免疫荧光和FISH技术检测海马CA1区NMDAR2B蛋白表达及基因转录的变化。结果:Morris水迷宫结果:丙泊酚组与对照组相比潜伏时间明显延长,穿越次数明显减少。免疫荧光结果:丙泊酚组与对照组比较NMDAR2B表达明显下降。FISH结果:丙泊酚组与对照组比较NMDAR2B表达明显下降。结论:丙泊酚麻醉引起老年大鼠短期认知功能障碍,其机制可能与海马CA1区NMDAR2B表达下调有关。
Objective：The effect of propofol anesthesia on learning memory and NMDAR2 B expression of CA1 in hippocampus were observed and the effect of propofol on postoperative cognitive dysfunction（POCD） was discussed. Methods：Male SD aged rats were randomly divided into control group and propofol group,with 20 rats in each group. Propofol group was given 50 mg·kg-1propofol by intraperitoneal injection. The control group was given the same dose of emulsion. The Morris water maze test was performed on 24 h after the end of anesthesia. The expression of NMDAR2 B protein and gene transcription of CA1 region were detected by immunofluorescence and FISH. Results：In Morris water maze test：compared with the control group,the latent period was signifi cantly prolonged,and the crossing times of propofol group were signifi cantly decreased. In immunofluorescence detection,the expression of NMDAR2 B was significantly decreased in propofol group. In FISH,compared with the control group,the expression of NMDAR2 B decreased significantly. Conclusion：Propofol anesthesia can induce a short-term cognitive dysfunction in aged rats,and its mechanism may be related to the down-regulation of NMDAR2 B expression in hippocampus CA1 region.
出处
《神经药理学报》
2015年第3期1-5,共5页
Acta Neuropharmacologica
基金
河北北方学院自然科学研究计划项目(No.120176)
