摘要
利用直线加速器全身均匀一次性照射昆明小鼠,建立放射损伤模型,经腹腔分别注射生理盐水(NS)、枸杞多糖(LBP)50、100和200 mg/kg,连续注射观察14天,计数小鼠外周血WBC、RBC、PLT及骨髓单个核细胞(BMNCs),检测BMNCs的细胞周期、凋亡率及BMNCs表面黏附分子CD49d和CD44的表达水平。结果表明,照射后小鼠表现为饮水、进食减少,行动缓慢、嗜睡,毛发凌乱,光泽性差,说明放射损伤模型建立成功;在相同时间点下,NS组与正常对照组相比,外周血WBC、RBC、PLT及BMNCs细胞计数明显减少(p<0.05)、BMNCs的细胞增殖能力明显减弱(p<0.05)、S期细胞比例显著减少(p<0.05)、G_0/G_1期细胞比例显著增加、BMNCs的细胞凋亡率明显增多(p<0.05)、黏附分子CD49d和CD44表达明显减少(p<0.05);而各LBP组与NS组相比,外周血各项指标、BMNCs细胞计数明显增多(p<0.05)、BMNCs细胞的增殖能力明显增强(p<0.05)、S期细胞比例显著增多(p<0.05)、G_0/G_1期细胞比例显著减少、细胞凋亡率明显减少(p<0.05)、黏附分子CD49d、CD44表达明显增加(p<0.05)。以上结果说明,LBP可能通过加速BMNCs细胞周期由G_0/G_1期向S期转换、降低BMNCs细胞凋亡率,提高放射损伤小鼠BMNCs细胞的增殖能力,进而上调细胞表面黏附分子CD49d和CD44的表达,来促进辐射损伤小鼠造血功能的恢复。
Lycium barbarum polysaccharide （LBP） has anti radiation damage effect. Animal models ofradiation injury were established by exposing Kunming mice to the linear accelerator X ray once. The irradiated mice were given intra-peritoneal injection with NS, LBP 50 mg/kg, LBP 100 mg/kg and LBP 200 mg/kg for 14 days respectively. The counts of WBC, RBC, PLT in peripheral blood and BMNCs were recorded BMNCs cell cycle cell apoptotic ratio and BMNCs surface adhesion molecule CDg9d and CD44 were detected to illuminate the molecular mechanism of LBP in sheltering radiation injured haematogenesis. Experimental results show that： After irradiation, the mice were observed drank and ate less than before, slow of movement, lethargy, messy hair and poor of glossiness, demonstrating the success of radiation damage model establishment; At the same time, NS group compared with the normal control group, Peripheral blood WBC, RBC, PLT and BMNCs count decreased significantly （p 〈 0.05）, the cell proliferation of BMNCs was significantly decreased （p 〈 0.05 ） , the proportion of cells in S phase significantly decreased （p 〈 0. 05 ）, G0/G1 phase cell percentage was significantly increased, the apoptotic rate of BMNCs cells increased significantly （p 〈 0.05）, cell adhesion molecule CD49d and CD4g expression was significantly reduced （p 〈 0.05） ; At the same time, compared with the NS groups, the results of LBP groups showed that the numbers of peripheral blood cells and BMNCs significant increased （p 〈 0. 05 ） , the cell proliferation of BMNCs was significantly increased （p 〈 0.05 ） , the proportion of cells in S phase increased significantly （p 〈 0. 05 ） , G0/G1 phase cell percentage was significantly decreased, the apoptotic rate was significantly lower （p 〈 0.05）, adhesion molecule CD49d and CD44 expression was significantly higher （p 〈 0.05）. In summary for BMNCs, LBP may enhance cell proliferation, accelerate cell cycle transition from Go/G1 phase to S phase, reduce apopt
出处
《辐射防护》
CAS
CSCD
北大核心
2016年第4期218-223,231,共7页
Radiation Protection
基金
重庆市自然科学基金(cstc2013jcyjA10072)
关键词
枸杞多糖
放射损伤
骨髓单核细胞
细胞周期
细胞凋亡
lycium barbarum polysaccharide （LBP）
radiation injury
bone marrow mononuclear（BMNC）
cell cycle
apoptosis
