摘要
为探讨在盐碱地区种植甘草对土壤理化性质及微生物功能多样性的影响。以未种植胀果甘草的裸地(CK)为对照,测定土壤的理化性质,并采用Bilog ECO微平板技术研究了人工栽培甘草对盐碱地微生物功能多样性的影响。研究结果表明:与裸地(CK)相比,种植甘草能显著提高盐碱土中全氮、碱解氮及有机质等养分的含量(P<0.05),其含量分别比裸地(CK)增加了38%、48%和33%,且土壤的电导率值与裸地(CK)相比降低了262%,达到显著水平(P<0.05)。甘草样地的AWCD值(反应土壤微生物活性)显著高于裸地(CK),在培养72 h后甘草样地的AWCD值比裸地(CK)显著增加了28%(P<0.05)。甘草样地中土壤微生物对糖类碳源的利用率与对照相比增加28%(P<0.05),其土壤微生物多样性亦显著高于裸地(CK)(P<0.05)。主成分分析表明,种植甘草后改变了土壤微生物的群落组成,导致此种差异的主要碳源为糖类和氨基酸类,其次是羟酸类及多聚物类。综上所述,在盐碱地种植甘草有利于提高土壤微生物代谢活性和土壤微生物群落功能多样性,因此人工栽培甘草是盐碱地生态恢复的一项有效措施。
The study aims to investigate the effect of planting licorice on physical and chemical properties and microbial community functional diversity in saline soil. Bare land （no planting Glycyrrhiza inflate） was set as control, and conventional methods were adopted in the experiment. Soil microbial community functional diversity was studied using BilogECO plates. Results showed that compared with that of control, the contents of total nitrogen, alkalystic nitrogen and organic matter of saline soil with licorice planting were significantly increased by 38%, 48% and 33%, respectively （P〈0.05）, electrical conductivity of soil significantly decreased by 262% （P〈0.05）. The results indicated that the Average Well Color Development （AWCD） of licorice growing soil was higher than that of bare land （CK）. Compared with that of bare land （CK）, AWCD of licorice growing soil significantly increased by 28% after cultured for 72 h. The utilization ratio of soil microorganism to sugar carbon sources in the licorice plot significantly increased by 28% （P〈0.05） and the soil microbial diversity was higher than that of bare land （CK） （P〈0.05）. Principal component analysis demonstrated that licorice changed the soil microbial community composition. Sugars and amino acids were the primary carbon sources to lead to the difference, followed by the hydroxyl acids and polymers category carbon sources. In a word, planting licorice could improve the activity and functional diversity of soil microbial community in saline soil. So licorice cultivation was a reasonable choice for ecological restoration in saline soil.
出处
《农学学报》
2016年第7期24-29,共6页
Journal of Agriculture
基金
国家自然科学基金项目"高盐环境下乌拉尔甘草叶片气孔泌盐与光合气体交换的利益冲突与权衡策略的研究"(31360047)
石河子大学重大科技攻关项目"高盐环境下乌拉尔甘草叶片气孔泌盐与光合气体交换的利益冲突与权衡策略的研究"(gxjs2012-zdgg06)
关键词
胀果甘草
盐碱地
土壤微生物
群落功能多样性
Glycyrrhiza inflata
Saline Soil
Soil Microorganism
Community Functional Diversity
