摘要
为建立超高效液相色谱-质谱/质谱法（UPLC-MS/MS）同时测定蔬菜中7种磺胺残留量的方法。样品用1%乙酸乙腈提取,以C18色谱柱分离待测物,采用多反应监测（MRM）离子扫描模式,外标法进行定量,线性良好,相关系数均大于0.999。结果显示：7种磺胺的检出限为0.000 4~0.000 6 mg/kg,样品添加回收率为67.5%~94.1%,相对标准偏差均少于5.3%（n=6）。该方法简单快捷、定量准确,可满足多种蔬菜中7种磺胺的残留检测要求。
The detectable method of 7 sulfanilamide in vegetables by UPLC-MS/MS spectrometry simultaneous has been established. The samples are initially extracted with 1% acetic acid acetonitrile. The eluate is separated by gradient elution with methanol-water solution of different ratio on C18（100 mm ×2.1 mm, 1.8 μm） column, and detected under positive multiple reactions monitoring（MRM）. The external standard calibration curves are used for quantitative analysis, the correlation coefficients is higher than 0.999. The results showed that the 7 sulfanilamide detection limits is 0.000 4 ~0.000 6 mg/kg. The average spiked recoveries ranged from 67.5%~94.1%, and the relative standard deviations（RSDs, n=6） is less than 5.3%. The method is quick, easy and effective, it can satisfy the demand for the determination of the 7 sulfanilamide residues in vegetables.
出处
《热带农业科学》
2016年第6期76-80,共5页
Chinese Journal of Tropical Agriculture
基金
典型抗生素蔬菜低累积品种筛选及污染控制关键技术研究与应用(No.2012108102043)
