摘要
目的研究真核翻译起始因子4γ1(eukaryotic translation initiation factor 4γ1,EIF4G1)对非小细胞肺癌细胞株A549细胞凋亡的抑制作用。方法使用RNAi技术下调A549细胞内EIF4G1的表达后,通过MTS法检测细胞生长增殖,采用流式细胞术(FCM)检测细胞凋亡,采用蛋白质印记(Western blot)技术检测下调EIF4G1后对A549细胞中与细胞凋亡相关的蛋白表达影响。结果下调A549细胞内EIF4G1的表达后,细胞生长受到明显抑制,细胞凋亡率也明显增加(P<0.05),且凋亡相关蛋白和对照组相比,FAS,P21和TRAF2的表达量明显增高(P<0.05)。结论 EIF4G1在A549细胞中表达降低后,凋亡相关蛋白FAS,P21和TRAF2的表达量升高,促进A549细胞凋亡。
Objective To investigate the effects of eukaryotic translation initiation factor 4γ1（EIF4G1） on apoptosis of human non-small cell lung cancer（NSCLC） cells.Methods EIF4G1 expression in NSCLC cells was knocked down by RNAi technology.Human NSCLC A549 cells were divided into three groups：blank control group,Si-EIF4G1 group and Si-NC group.The proliferation of the A549 cell was analyzed by M TS and the apoptosis rates were detected by flowcytometry.EIF4G1 protein and apoptosis-related proteins in A549 cells were detected by Western blot.Results The proliferation of A549 cells in Si-EIF4G1 group were decreased compared with Si-NC group.Flowcytometry showed that cell apoptosis in Si-EIF4G1 group was increased（4.65% ± 6.12%） compared to NC（1.26% ± 2.60%） and Si-NC groups（3.88% ± 3.52%）.Western-blot showed that the expressions of apoptosis-related proteins FAS,P21 and TRAF2 were increased（P〈0.05）.Conclusion EIF4G1 can inhibit apoptosis of A549 by regulating the expression levels of FAS,P21 and TRAF2.
出处
《同济大学学报（医学版）》
CAS
2016年第3期50-55,共6页
Journal of Tongji University(Medical Science)
基金
上海市浦东新区科委创新基金(PKJ2015-Y16)
上海市浦东新区卫生系统领先人才培养计划(PWRL2014-01)
