摘要
目的探讨体外培养条件下异丙酚对高糖(HG)诱导人脐静脉血管内皮细胞(HUVECs)氧化应激及细胞黏附分子的影响。方法将新鲜脐带中分离培养的HUVECs分为6组:C组为正常糖浓度(5.5 mmol·L^(-1))组,P组为异丙酚(25μmol·L^(-1))组,HG组为高糖(25 mmol·L^(-1))组,HG+P1组为高糖(25 mmol·L^(-1))+异丙酚(1μmol·L^(-1))组,HG+P5组为高糖(25 mmol·L^(-1))+异丙酚(5μmol·L^(-1))组,HG+P25组为高糖(25 mmol·L^(-1))+异丙酚(25μmol·L^(-1))组,处理8 h后采用分光光度比色法检测超氧化物歧化酶(SOD)活力,DCFH-DA荧光探针法检测内皮细胞内的活性氧(ROS)含量,实时定量PCR、Western Blot检测细胞间黏附分子^(-1)(ICAM^(-1))的表达。结果与C组比较,HG组SOD活力明显下降,ROS、ICAM^(-1)明显增高,而异丙酚在适当浓度可干预上述变化。结论异丙酚通过抑制高糖诱导的HUVECs的氧化应激和黏附分子表达,对改善糖尿病血管炎症病变进展起一定的作用。
Objective To investigate the effect of propofol on high glucose（HG）-induced oxidative stress and intercellular adhesion molecule（ICAM）-1 expression in human umbilical vein endothelial cells（HUVECs） and the underlying mechanisms. Methods HUVECs were isolated from the human umbilical cords from normal pregnancies and exposed to 6 media： a C group： 5.5 mmol·L-1 glucose used as the normal glucose induction model; a P group： 25 μmol·L-1 propofol; a HG group： 25 mmol·L-1 glucose; a HG ＋ P1 group： 25 mmol·L-1 glucose and 1 μmol·L-1 propofol; HG ＋ P5 group： 25 mmol·L-1 glucose and 5 μmol·L-1propofol; a HG ＋ P25 group： 25 mmol·L-1 glucose and 25 μmol·L-1 propofol. After incubation for 8 h, the cultured medium was collected to measure the superoxide dismutase（SOD） activity by spectrophotometer method and the cells were harvested for Western blot and for the real-time polymerase chain reaction to observe the expression of ICAM-1. The generation of intracellular reactive oxygen species（ROS） was assessed with fluorescent probe dichlorofluorescein diacetate（DCFH-DA）. Results Compared with the normal glucose group, high glucose significantly reduced the SOD activity（P 〈 0.05） and increased the levels of ROS（P 〈 0.05） and ICAM-1 expression（P 〈 0.05）. Propofol treatment concentration-dependently attenuated HG-induced oxidative damage on HUVECs. Conclusion Vascular inflammatory responses induced by HG are critical events underlying the development of diabetic complications. Propofol possesses obvious therapeutic effects against diabetic complications and atherosclerosis.
出处
《中南药学》
CAS
2016年第6期581-585,共5页
Central South Pharmacy
基金
国家自然科学基金项目(No.81201448)
关键词
异丙酚
高糖
人脐静脉内皮细胞
氧化应激
细胞间黏附分子-1
超氧化物歧化酶
活性氧
propofol
high glucose
human umbilical vein endothelial cells
oxidative stress
intercellular adhesion molecule-1
superoxide dismutase
reactive oxygen species
