摘要
目的探讨miR-146a和LIN52在晚期胃癌中的表达情况及其与临床预后的相关性。方法提取93例胃癌组织标本的总RNA，采用实时定量聚合酶链反应（RT—qPCR）方法检i测miR-146a的相对表达量，免疫组织化学方法检测LIN52表达情况，并分析其临床诊断特异性及其与肿瘤TNM分期、淋巴结转移、分化程度以及肿瘤疗效和预后等临床病理因素的关系。结果miR-146a在胃癌组织中的表达与淋巴结转移呈负相关（P〈0．05）。miR-146a的表达与患者预后有相关性（P〈0．01）。miR-146a高表达患者的生存率提高，而LIN52高表达患者的生存率则降低（P〈0．05）。受试者工作特征曲线分析显示miR-146a用于诊断胃癌的敏感度和特异度分别为94．1％和61．5％。结论miR-146a在胃癌中作为抑癌基因与LIN52存在负相关，其高表达患者的化疗有效率可提升。miR-146a可能作为胃癌诊断和预后判断的分子标志物。
Objective To explore the expression of miR-146a and its target gene LIN52 in advanced gastric cancer and their potential impact on clinical prognosis. Methods Total RNAs were extracted from 93 gastric cancer tissues and their corresponding adjacent non-tumor tissues to quantify the relative expression of miR-146a by using real-time quantitative PCR （RT-qPCR）. Expression of LIN52 was detected in tumors and normal tissues by immunohistochemistry. Correlation analysis was assessed between the expression of miR-146a and LIN52 and clinicopathological parameters, including clinical diagnostic specificity, tumor TNM staging, lymph node metastasis, differentiation grade, curative effect and prognosis of gastric cancer. Results The expression of miR-146a in gastric carcinoma was negatively correlated with lymph node metastasis （P 〈 0.05）. The expression of miR-146a had a significant correlation with the prognosis of the patients （P 〈 0.01）. The patients with high expression of miR-146a had higher survival rate （P 〈 0.05）, but the patients with high expression of LIN52 had lower survival rate （P 〈 0.05）. The receiver operating characteristic curve regression analysis showed that sensitivity and specificity of miR-146a were 94.1% and 61.5 % to diagnose gastric cancer. Conclusions As a tumor suppressor gene in gastric cancer, miR-146a has significantly negative correlation with LIN52. High expression of miR-146a in the gastric cancer tissue might be associated with improved treatment efficacy of chemotherapy, suggesting that miR-146a may be a molecular marker for the diagnosis and prognosis of gastric cancer.
出处
《肿瘤研究与临床》
CAS
2016年第6期388-393,共6页
Cancer Research and Clinic
基金
国家自然科学基金（U1504820）
