摘要
矢车菊素-3-芸香糖苷(C3R)是桑椹花青素的主要成分,研究从成熟桑椹中提取C3R对链脲佐菌素(STZ)诱导人源性胰岛β细胞凋亡的抑制作用及可能的调控机制。采用MTT法及流式细胞计数检测发现:桑椹C3R对正常胰岛β细胞株INS-1具有极显著的促增殖作用(P<0.01);50μg/m L桑椹C3R液处理STZ诱导凋亡模型胰岛β细胞株INS-1 48 h后,其早期凋亡、晚期凋亡和坏死的细胞数量极显著下降(P<0.01)。以经桑椹C3R液处理的STZ诱导凋亡模型胰岛β细胞的c DNA为模板,通过qRT-PCR检测细胞中的几个凋亡相关蛋白基因的转录水平变化,其中抗凋亡蛋白基因Bcl-x L的mRNA转录水平极显著上调(P<0.01),促凋亡蛋白基因Bad、Bax及Caspase 8、Caspase 6基因的mRNA转录水平极显著下调(P<0.01)。研究结果表明,桑椹C3R对STZ诱导的胰岛β细胞凋亡具有明显的抵抗作用,由此也证实了桑椹花青素的药用开发价值。
Cyanidin-3-rutinoside（ C3R） is the main component of mulberry anthocyanins. In this paper,the inhibitory effect and its underlying regulatory mechanism（ s） of C3 R from mature mulberry fruit on human-derived pancreatic cell apoptosis induced by streptozotocin（ STZ） was studied. After measured by MTT and flow cytometry,the results showed that C3 R could significantly increase the proliferation of pancreatic β-cell line INS-1（ P 〈 0. 01）. At 48 h after being treated with 50 μg / m L mulberry C3 R,the number of early apoptotic,late apoptotic,and necrosis cells was extremely significantly decreased（ P 〈 0. 01）. By using c DNA of STZ-induced pancreatic β-cell treated with mulberry C3 R as template,the expression variation of apoptosis-related protein genes was detected by real-time fluorescent quantitative PCR（ qRTPCR） method. The results showed that the mRNA transcriptional level of anti-apoptosis protein gene Bcl-x L was extremely significantly up-regulated（ P 〈 0. 01）,while the mRNA transcriptional levels of pro-apoptotic protein genes Bad,Bax,Caspase 8 and Caspase 6 were extremely significantly down-regulated（ P 〈 0. 01）. These results indicated that C3 R from mulberry fruit has obvious inhibitory effect on pancreatic β-cell apoptosis model induced with STZ,which provides a scientific reference for medicinal utilization of mulberry anthocyanins.
出处
《蚕业科学》
CAS
CSCD
北大核心
2016年第3期507-512,共6页
ACTA SERICOLOGICA SINICA
基金
浙江省蚕桑产业科技创新团队项目(No.2011R50028)
现代农业产业技术体系建设专项(No.CARS-22)
浙江省蚕桑农业新品种选育重大科技专项(No.2012C12910)
