摘要
ABA 8＇羟化酶是脱落酸（abscisic acid,ABA）分解代谢中的关键酶基因之一。采用同源检索的方法从太子参转录组数据库中获得7个ABA 8＇羟化酶（abscisic acid 8＇-hydroxylase）基因家族成员,对各家族成员进行转录分析组数据以及ABA 8＇羟化酶关键基因ABA8ox1编码区（coding sequence,CDS）的克隆与生物信息学分析。ABA8ox1基因CDS全长1 401 bp,编码480个氨基酸残基,等电点为8.55,相对分子质量为53 k Da,跨膜分析显示其为膜蛋白,1-21个氨基酸残基位于胞内,22-466个氨基酸残基位于胞外。转录组数据分析及定量PCR技术共同证明ABA8ox1在块根的韧皮部及须根中有较高的表达。多序列比对及聚类分析显示与其他植物CYP707A蛋白具有较高的同源性,且与模式植物拟南芥中ABA分解关键基因At CYP707A1和At CYP707A3聚为一类。该研究为太子参块根膨大及对逆境胁迫响应的分子机制奠定基础。
Abscisic acid 8＇-hydroxylase was one of key enzymes genes in the metabolism of abscisic acid（ ABA）. Seven menbers of abscisic acid 8＇-hydroxylase were identified from Pseudostellaria heterophylla transcriptome sequencing results by using sequence homology. The expression profiles of these genes were analyzed by transcriptome data. The coding sequence of ABA8ox1 was cloned and analyzed by informational technology. The full-length c DNA of ABA8ox1 was 1 401 bp,with 480 encoded amino acids. The predicated isoelectric point（ p I） and relative molecular mass（ MW） were 8. 55 and 53 k Da,respectively. Transmembrane structure analysis showed that there were 21 amino acids in-side and 445 amino acids out-side. High level of transcripts can detect in bark of root and fibrous root. Multi-alignment and phylogenetic analysis both show that ABA8ox1 had a high similarity with the CYP707 As from other plants,especially with At CYP707A1 and At CYP707A3 in Arabidopsis thaliana. These results lay a foundation for molecular mechanism of tuberous root expanding and response to adversity stress.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2016年第13期2397-2403,共7页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81460579)
贵州省社会发展攻关项目(黔科合SY[2015]3030)
关键词
太子参
脱落酸
降解
ABA
8'羟化酶
表达分析
Pseudostellariae Radix
abscisic acid
degradation
abscisic acid 8＇-hydroxylase
expression analysis
