摘要
目的探讨不同蛋白水平下补充丝氨酸对大鼠高同型半胱氨酸血症的干预效果。方法将48只健康Wistar大鼠随机分为低蛋白组〔10%酪蛋白(10C),10C+0.75%蛋氨酸(10CM),10CM+2.5%丝氨酸〕和高蛋白组〔40%酪蛋白(40C),40C+0.75%蛋氨酸(40CM),40CM+2.5%丝氨酸〕。实验喂养14 d后处死动物,采集血液、肝脏样品,用于测定血液生化指标及肝脏蛋氨酸中间代谢物和酶学指标。结果丝氨酸的添加显著抑制了低蛋白、高蛋白饲料中蛋氨酸诱导的同型半胱氨酸浓度的升高,同时降低了肝脏S-腺苷甲硫氨酸浓度。丝氨酸的补充恢复了由低蛋白饲料中添加蛋氨酸导致的肝脏丝氨酸浓度的降低。结论丝氨酸对低蛋白饲料诱导的高同型半胱氨酸血症的干预效果显著强于高蛋白组,显著抑制了由蛋氨酸添加诱导的高同型半胱氨酸血症的发生。
Objective To investigate the effects of dietary protein in different level on hyperhomocysteinemia by Serine addition. Methods A total of 48 Wistar rats were fed low protein （10C） and high protein （40C） diets with 0.75% L-Methionine and 2.5% Serine for 14 days, and they were killed by decapitation to obtain blood and livers for biochemical analysis. Results The Met-induced high concentration of Hcy in rats was significantly suppressed by supplementation with serine in low and high protein dietary. At the same time, hepatic concentration of SAM decreased by supplementation with serine. The decrease in hepatic serine concentration was recovered by supplementation with serine in low protein dietary. Conclusion The results indicated that serine can completely suppress the hyperhomocysteinemia induced by Met, and Serine also exhibiter stronger inhibitory effect in the low protein dietary than against high protein dietary.
出处
《解放军预防医学杂志》
CAS
2016年第3期323-326,共4页
Journal of Preventive Medicine of Chinese People's Liberation Army
基金
中国营养学会营养科研基金-伊利专项科研基金(No.2014-37 CNS-YL)
