摘要
背景:将骨形态发生蛋白与中空多孔钛合金复合,可提升材料与周围骨组织的亲和力。目的:观察骨形态发生蛋白2对中空多孔金属假体支架内骨髓间充质干细胞生长及成骨分化的影响。方法:将第3代SD大鼠骨髓间充质干细胞直接接种于中空多孔金属假体支架上,分别以含0,0.001,0.01,0.06,0.1 g/L骨形态发生蛋白2的DMEM培养基培养,培养0,6,12,24,48 h,采用MTT法检测细胞黏附情况;培养18 d,茜素红染色检测细胞成骨分化能力。将Transwell培养池置于中空多孔金属假体支架上,上室加入5×108 L-1的骨髓间充质干细胞悬液,下室分别加入含0,0.001,0.01,0.06,0.1 g/L骨形态发生蛋白2的DMEM培养基,培养0,6,12,24,48 h后检测细胞迁徙能力。结果与结论:1培养6-48 h,不同质量浓度骨形态发生蛋白2呈时间依赖性促进骨髓间充质干细胞的黏附;2培养18 d,经不同质量浓度骨形态发生蛋白2干预后,骨髓间充质干细胞由梭形改变为多角形,细胞呈多层性、重叠性排列,形成大量钙化结节,茜素红染色呈鲜红色;3培养6-48 h内,骨形态发生蛋白2可促进骨髓间充质干细胞的迁徙,且呈浓度、时间依赖性;4结果表明:骨形态发生蛋白2可增强中空多孔金属假体支架上骨髓间充质干细胞在的黏附能力、成骨分化能力与迁徙能力。
BACKGROUND： Bone morphogenetic protein in combination with hol ow porous titanium al oy can improve the affinity with surrounding bone tissues. OBJECTIVE： To observe the effects of bone morphogenetic protein 2 on growth and osteogenic differentiation of bone marrow mesenchymal stem cel s cuftured on a hol ow porous metal prosthesis scaffold. METHODS： Passage 3 Sprague-Dawley rat bone marrow mesenchymal stem cel s were directly inoculated onto a hol ow porous metal prosthesis, and then the scaffold was cultured in DMEM medium containing 0, 0.001, 0.01, 0.06 and 0.1 g/L bone morphogenetic protein 2, respectively. At 6, 12, 24 and 48 hours after inoculation, cel adhesion was detected by MTT assay. Cel osteogenic differentiation was detected by alizarin red staining at 18 days. Besides, Transwel culture was put on the scaffold, and 5x108/L bone marrow mesenchymal stem cel s were added into the upper chamber, and DMEM medium containing 0, 0.001, 0.01, 0.06 and 0.1 g/L bone morphogenetic protein 2 were added into the lower chamber to observe cel migration capability after 0, 6, 12, 24 and 48 hours culture. RESULTS AND CONCLUSION： After 6-48 hours of inoculation, different mass concentrations of bone morphogenetic protein 2 promoted adhesion of bone marrow mesenchymal stem cells in a time-dependent manner. After 18 days of inoculation, bone marrow mesenchymal stem cells induced by different mass concentrations of bone morphogenetic protein 2 changed from fusiform to polygon, and arranged in a multilayer and overlapped form. Numerous calcified nodules could be found, which were stained red by alizarin red. Additionally, within 6-48 hours of culture, bone morphogenetic protein 2 could promote the migration of bone marrow mesenchymal stem cells in a concentration-and time-dependent manner. In conclusion, bone morphogenetic protein 2 can enhance the adhesion, osteogenic differentiation and migration of bone marrow mesenchymal stem cells cultured on the hollow porous metal prosthesis.
出处
《中国组织工程研究》
CAS
北大核心
2016年第25期3673-3679,共7页
Chinese Journal of Tissue Engineering Research
