摘要
目的探讨同步检测27个STR基因座多态性及法医学应用价值。方法用Power PlexFusion 6C荧光标记系统对274份广东汉族无关个体的DNA进行PCR扩增,在3130XL遗传分析仪上进行电泳分析。用Gene MapperID-X软件分析基因型,用Power Stats v1.2软件分析群体遗传学参数。并观察该系统对微量及混合检材的检验效果。结果 23个常染色体STR基因座遗传多态性高,在广东汉族人群的累积个人识别率为1-2.3×10-28,累积非父排除率为0.999 999 999。DYS391、DYS570和DYS576的GD值分别为0.481、0.791和0.751,共检出58种单倍型,单倍型多态性为0.337。微量检材STR基因座检出率为93.33%,混合检材Y-STR基因座检出率为96.67%。结论 Power PlexFusion 6C系统遗传多态性高,可以用于亲缘鉴定、个体识别以及数据库建设。
Objective To explore polymorphism and forensic application by detecting 27 STR locus simultaneously. Methods The DNA of the samples was extracted by magnetic beads, amplified with PowerPlex Fusion 6C ampli- fication Kit with 6-dye fluorescences labeling, and analyzed with GeneMapper@ ID-X software. The Population ge- netics parameters were evaluated with Power States v 1.2 software. In addition, The effect of system about trace and mixture sample was observed. Results The twenty-three autosomal STR loci were of high polymorphism in Guangdong Han population. TDP was 1 - 2.3 x 10-2s, TPE was 0. 999 999 999. DYS391, DYS570 and DYS576 loci GD value was 0. 481,0. 791and 0. 751, respectively. Three Y-STR in 172 unrelated individuals male 58 kinds of haploid type were detected, haplotype polymoqohism of 0. 337. The trace and mixture samples STR detection rate were 93.33% and 96. 67%. Conclusions PowerPlex Fusion 6C is a high genetic polymorphism system, which can be used for kinship analysis, identification of the individual, and database construction
出处
《基础医学与临床》
CSCD
2016年第7期969-975,共7页
Basic and Clinical Medicine
基金
广东省科技计划(2013B021500010)
