摘要
目的：建立以高效液相色谱法同时测定羚羊清肺散中绿原酸、栀子苷、芍药苷和甘草苷的含量的方法。方法：采用双波长HPLC法,Kromasil C18色谱柱（4.6 mm×250 mm,5μm）,流动相乙腈-0.1%磷酸水梯度洗脱,流速1.0 m L·min-1,检测波长327 nm（绿原酸）,237 nm（栀子苷、芍药苷、甘草苷）。结果：绿原酸、栀子苷、芍药苷和甘草苷4种成分进样量分别在1.579-78.96μg（r=0.999 8）,1.261-63.04μg（r=1）,0.364-18.2μg（r=0.999 9）,0.329 6-16.48μg（r=0.999 9）与峰面积线性关系良好;平均回收率分别为97.6%,97.6%,97.1%和99.8%,RSD分别为1.0%,0.7%,1.2%和1.3%。结论：该方法简便、快速、准确,可用于羚羊清肺散的质量控制。
Objective： To establish a HPLC method for simultaneously determining the contents of chlorogenic acid, gardenoside, paeoniflorin and liquiritin in Lingyang Qingfei powder. Method： A double wavelength HPLC method was performed on Kromasil C18column（ 4. 6 mm × 250 mm,5 μm） with acetonitrile and0. 1% phosphoric acid solution as the mobile phase for gradient elution. The flow rate was set at 1. 0 m L·min- 1and the column temperature was 28 ℃. The detector wavelength was 327 nm for chlorogenic acid and 237 nm for gardenoside,paeoniflorin and liquiritin. Result： The chlorogenic acid,gardenoside,paeoniflorin and liquiritin showed good linear relationship with peak area at the sample size of 1. 579-78. 96 μg（ r = 0. 999 8）,1. 261-63. 04 μg（ r = 1）,0. 364-18. 2 μg（ r = 0. 999 9）,and 0. 329 6-16. 48 μg（ r = 0. 999 9） respectively. The average recovery rate was 97. 6%,97. 6%,97. 1% and 99. 8% respectively for the above components, with RSD of 1. 0%, 0. 7%, 1. 2% and 1. 3%, respectively. Conclusion： The method is simple, rapid and accurate,and can be used for the quality control of Lingyang Qingfei powder.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2016年第13期77-80,共4页
Chinese Journal of Experimental Traditional Medical Formulae
