摘要
为研究马来西亚红罗非鱼(Oreochromis mossambicus×O.niloticus)幼鱼的耐盐性能,进行了急性和慢性盐度胁迫对体质量为(27.27±2.03)g的红罗非鱼幼鱼存活及肌肉组分的影响试验,急性盐度胁迫试验设置7个盐度(0、5、10、15、20、25、30),分别记为S_0、S_5、S_(10)、S_(15)、S_(20)、S_(25)、S_(30)组,试验周期为5d,慢性盐度驯化试验设置5个盐度(0、5、10、15、20),分别记为G_0、G_5、G_(10_、G_(15)、G_(20)组,试验周期为25 d。结果表明:急性盐度胁迫试验中,红罗非鱼开始死亡盐度为15,随着盐度的升高死亡率急剧上升,并在盐度为25及以上时达到100%,96 h半致死盐度为18;急性盐度胁迫下,红罗非鱼肌肉的水分、粗脂肪和粗蛋白质含量在各盐度中均未有显著性差异(P>0.05),而S_(30)组鱼肌肉灰分含量显著高于盐度15及以下组(P<0.05);慢性盐度胁迫试验中,红罗非鱼从盐度10开始死亡,并且累计死亡率随盐度的升高而上升,但在最高盐度20时,25 d的累计死亡率仍低于40%;慢性盐度驯化下,G_5组鱼肌肉粗脂肪含量显著高于G_(15)组(P<0.05),粗蛋白质含量显著低于G_(15)和G_(20)组(P<0.05),灰分含量显著高于G_(10)、G_(15)和G_(20)组(P<0.05)。研究表明,马来西亚红罗非鱼可以直接在低盐度的咸淡水中生活,经过盐度驯化后该鱼具有在较高盐度海水中生活的可能性,适宜的盐度和养殖驯化方式可改善红罗非鱼的肌肉品质。
The objective of this study was to investigate salt tolerance and muscle component of juvenile Malaysia red tilapia （ Oreochromis mossambius × O. niloticus） in acute salinity stress and salinity acclimatization. In the acute salinity stress trial,10 fish with initial body weight of （27. 27±2. 03） g were exposed to a salinity of 0 （ S0）, 5 （S5）, 10（S10 ）,15（S15）,20（S20）, 25（S25）or 30（S30） with 3 replicates for five days. The results showed that fish death was first observed at a salinity of 15 , and that the mortality was increased dramatically with increasing level of salinity, with 100% mortality at a salinity of 25 and 30, and median lethal salinity- 96 h （MLS-96） at 18. Under the acute salinity stress, there were no significant differences in moisture, crude lipid and crude protein of Malaysia red tilapia muscle （P〉0.05） , while there was significantly higher ash in S30 group than that in other groups. In salinity acclimatization trial, 15 Malaysia red tilapia juveniles were acclimatized to a salinity of 0（G0） , 5 （G5）, 10（GI0）, 15（G,5） or 20（G20） with 3 replicates per group for 25 days. The results showed that fish death was first observed at a salinity of 10 and that the mortality was increased with increasing level of salinity, with less than 40% of mortality in G20. There was significantly higher muscular crude lipid in G5 than that in G15（P 〈0. 05）. However, the muscular crude protein was found to be significantly lower in G5 than that in G15 and G20（ P〈0. 05）. The muscular ash in G5 was higher than that in G10, G15 and G20（ P〈0.05）. The findings indicate that Malaysia red tilapia can directly be transformed and live in low salinity water and possibly survive in high salinity water after sa-linity acclimatization which may improve the muscle component.
出处
《大连海洋大学学报》
CAS
CSCD
北大核心
2016年第3期280-284,共5页
Journal of Dalian Ocean University
基金
中央级公益性科研院所基本科研业务费专项(2015JBFM07)
