摘要
采用扫描电镜和石蜡切片方法观察野生型烟草和转基因不育烟草的花药及其横切结构,研究真姬菇热激蛋白HmHSP70导致烟草雄性核不育的机理,结果表明:转基因不育烟草花粉的小孢子和花药壁发育异常,小孢子无法进行有丝分裂而降解,而绒毡层细胞和中层细胞过度分裂不降解,药室遭到严重挤压,是导致花粉败育的主要原因。
In order to research the mechanism of genic male sterility,scanning electron microscope and paraffin section method were adopted in this study to observe the structure of anther and its transverse section in transgenic sterile tobacco and wild--type control. Results showed that microspore of transgenic sterility tobacco development anomalies and non division, but the cells of anther tapetal and middle layer splitted excessively and non degradable, then the Pollen chamber was squeezed severely were the main reasons of pollen sterility.
出处
《青岛农业大学学报（自然科学版）》
2016年第1期16-19,共4页
Journal of Qingdao Agricultural University(Natural Science)
基金
国家自然科学基金项目(31172012/C1506)
烟草行业烟草基因资源利用重点实验室开放课题(TB201110)
关键词
花粉败育
转基因烟草
石蜡切片
扫描电镜
pollen sterility
transgenic tobacco
paraffin section
electron microscopy
