摘要
草甘膦是全球用量最大的除草剂之一,但它的大量使用仍然带来了一定的环境风险。该文主要探讨了低浓度(2 mg/L)和高浓度(10 mg/L)草甘膦对铜绿微囊藻的毒性效应,并且深入微囊藻的抗坏血酸与谷胱甘肽抗氧化系统,深入研究了草甘膦对DHAR和GR酶的活性的影响,并分析了草甘膦存在时微囊藻细胞中的还原型As A与GSH的含量变化。结果表明:与10 mg/L草甘膦导致的微囊藻的生物量减少,氧化损伤加重不同,2 mg/L草甘膦会刺激微囊藻的生长和蛋白合成。这可能是由于2 mg/L草甘膦会刺激微囊藻体内的DHAR和GR酶的活性,从而再生更多的还原型AsA与GSH,从而减轻了草甘膦诱导的微囊藻的氧化损伤。
Glyphosate is one of the most widely used herbicides in the world.However,its environmental risk always exists during its use.In order to investigate the toxicity of glyphosate and the possible mechanisms,2 mg/L and 10 mg/L concentrations of glyphosate were added separately in the Microcystis aeruginosa medium and the activities of DHAR and GR were analyzed, as well as the contents of reduced AsA and GSH.The results showed that glyphosate stimulated the growth and protein synthesis at the concentration of 2 mg/L ,while the biomass was reduced and the oxidative damage was aggravated when the glyphosate concentration was 10 mg/L.The possible reasons reside in the fact that increased activities of DHAR and GR would regenerate more reduced AsA and GSH, which could alleviate the oxidative damage induced by the herbicide when the concentration of glyphosate was 2 mg/L.
出处
《现代农业科技》
2016年第10期85-87,89,共4页
Modern Agricultural Science and Technology
基金
国家级大学生创新训练计划项目:灭生性除草剂对水华爆发的影响及原因研究(201410449037)
滨州学院博士学位人员及具有硕士学位的高级职称人员科研启动项目:禾草灵与壬基酚对水华蓝藻铜绿微囊藻的联合毒性研究(2013Y16)
