摘要
目的:研究氧化应激对原代培养乳鼠心房肌细胞网腔钙结合蛋白(calumenin)、内质网应激及信号传导通路作用,探讨氧化应激与心房肌细胞网腔钙结合蛋白、内质网应激及其凋亡信号传导通路相关性。方法:实验分2组:对照组和氧化应激组。原代培养乳鼠心房肌细胞,氧化应激组在培养的原代心房肌细胞中加入终浓度为100μmol/L的H2O2培养2h,检测氧化和抗氧化指标的变化。ELASE法检测2组心房肌细胞超氧化物歧化酶(SOD)活力、丙二醛(MDA)及还原型谷胱甘肽(GSH)含量。Western bloting检测2组心房肌细胞GRP78、GRP94、chop、IRE1及caspase-3、caspase-9、caspase-12表达。结果:1与对照组比较,氧化应激组心房肌细胞SOD活力下降,GSH含量下降,MDA含量增加(P<0.01)。2与对照组比较,氧化应激组心房肌细胞calumenin表达减少(P<0.01),而GRP78、GRP94、chop、IRE1及caspase-3、caspase-9、caspase-12表达增加(P<0.01)。结论:氧化应激反应能引起心房肌细胞calumenin表达减少,这可能介导心房肌细胞内质网应激反应并激活其凋亡信号传导通路表达,导致心房肌细胞凋亡,参与心房结构重构引发房颤。
Objective：To investigate the effect of oxidative stress on the expression of calumenin,endoplasmic reticulum stress and signal transduction pathway in the primary cultured suckling mouse atrial myocytes,and to explore the correlation between oxidative stress and the expression of calumenin,endoplasmic reticulum stress and apoptosis signal transduction pathway.Method：The suckling mouse myocardium of primary culture were randomly divided into control group and the oxidative stress group.Each group was cultured 2hwith the concentration of100umol/L H2O2,then detected the changes of oxidation and antioxidation indexs.The activity of superoxide dismutase（SOD）and the content of malondialdehyde（MDA）and glutathione（GSH）in 2groups were detected by ELASE assay.The expression of GRP78,chop,IRE1,Caspase-3,caspase-12,GRP94 and caspase-9were detected by Western bloting.Result：Compared with the control group,the SOD activity and the GSH content of the atrial myocytes in the oxidative stress group were decreased,and the content of MDA was increased（P〈0.01）.Compared with the control group,the expression of calumenin was decreased in the oxidative stress group（P〈0.01）,while the expression of GRP94,chop,IRE1 and Caspase-3,caspase-9,GRP78,and caspase-12 were increased.Conclusion：Oxidative stress can induce the expression of calumenin in atrial myocytes,which may mediate the endoplasmic reticulum stress reaction and activate the expression of the apoptosis signal transduction pathway.This leads to the apoptosis of atrial myocytes and involves in atrial structural remodeling of atrial fibrillation.
出处
《临床心血管病杂志》
CAS
CSCD
北大核心
2016年第6期611-613,共3页
Journal of Clinical Cardiology
关键词
氧化应激
网腔钙结合蛋白
内质网应激
凋亡
oxidative stress
calumenin
endoplasmic reticulum stress
apoptosis
