摘要
目的：建立金精固体饮料的HPLC指纹图谱，为有效评价其质量提供参考。方法采用HPLC法对10批金精固体饮料样品进行测定，以Kromasil C18（4.6 mm×250 mm，5μm）为色谱柱，流动相为乙腈-0.1%磷酸溶液，梯度洗脱，检测波长327 nm，流速1.0 ml/min，柱温35℃。采用中药色谱指纹图谱相似度评价软件对结果进行评价。结果建立了金精固体饮料的 HPLC 指纹图谱，并标定了包括绿原酸在内的12个共有峰，10批样品的相似度均＞0.9。结论该法操作简便可靠，所建立的指纹图谱重现性良好，可作为控制和评价金精固体饮料质量的方法。
Objective To establish an HPLC fingerprint ofJinjing solid beverage,and provide experimental evidence of evaluating its quality.Methods Ten batches ofJinjing solid beverage were analyzed by HPLC under the gradient elution condition. A Kromasil C18 column（4.6 mm×250 mm, 5μm） was used, and the flow phase was acetonitrile-H2O（acidified to 0.1% with phosphoric acid） with gradient elution, and the detection wavelength was 327 nm, and the flow rate was 1.0 ml/min, and the column temperature was 35℃. The data were evaluated by the similarity evaluation software for TCM fingerprint.Results The HPLC fingerprint ofJinjing solid beverage were established. Twelve common peaks including chlorogenic acid were identified with similarity of more than 0.9.Conclusion HPLC method is a reliable, available and quick method, that provides a means for controlling and evaluating the quality ofJinjing solid beverage.
出处
《国际中医中药杂志》
2016年第6期546-549,共4页
International Journal of Traditional Chinese Medicine
基金
重庆市科技支持项目（cstc2014fqzktjcsf10019）
关键词
色谱法
高压液相
DNA指纹法
绿原酸
金精固体饮料
Chromatography,high pressure liquid
DNA Fingerprinting
Chlorogenic acid
Jinjing solid beverage
