摘要
犬流感(CI)是由犬流感病毒(CIV)感染犬引起的传染性疾病,感染犬主要表现为急性呼吸道症状。为了探究犬microRNA let-7a对CIV诱导细胞凋亡的调控作用,在体外用CIV对MDCK细胞进行感染,通过AO/EB染色和流式细胞术检测是否能引起细胞凋亡,并用荧光定量PCR测定let-7a的转录水平,最后通过转染let-7a来评价其对于细胞凋亡的影响。结果表明,CIV能够引起MDCK细胞发生凋亡且被感染细胞内let-7a转录下调,而过转录细胞内let-7a则能够有效地抑制病毒诱导的凋亡。研究结果显示,犬let-7a在CIV感染细胞中起着抗凋亡作用,而let-7a的低转录促进了细胞凋亡,并且这一机制可能在CIV感染犬的呼吸道损伤中扮演了一定的角色。
Canine influenza（CI）is an infectious disease caused by canine influenza virus（CIV）infection,which is mainly manifested as acute respiratory symptoms.In order to explore the effect of let-7a on CIV induced cells apoptosis,MDCK cells were infected by CIVin vitro,and AO/EB staining and flow cytometry were used to detect cells apoptosis,and the transcription of let-7a was also determined by real time PCR,and the effect of let-7a on cell apoptosis was evaluated by the transfection of let-7a.The results showed that CIV could induce apoptosis of MDCK cells and downregulate the transcription of let-7a in infected cells,and over-transcription of let-7a could effectively inhibit the virus induced apoptosis.This study showe that let-7a plays an anti-apoptotic role in CIV infected cells,and the low transcription of let-7a promotes apoptosis,and this mechanism may play a role in the respiratory tract injury of CIV infected dogs.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2016年第6期1247-1252,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金(31372448)
公益性行业(农业)科研专项(201303042)
广东省自然科学基金(S2013020013858)
广东省促进科技服务业发展计划项目(2013B040200032)
广东省科技计划项目(2013B020202001
2013B020224001)
广东省兽医临床重大疾病综合防控重点实验室(2013A061401013)
