摘要
该文采用超声法制备一种新型可降解、生物相容性良好的负载姜黄素(curumin,Cur)红细胞膜纳米粒子(Cur-RBCNPs)。通过比较不同参数制备所得纳米粒子的粒径来优化Cur-RBC-NPs的制备条件,并考察其形态、粒径分布、稳定性、体外释放特性、细胞对纳米粒子的摄取及体外抗肿瘤效果等。结果表明,所制得姜黄素纳米粒子为较规则的圆球形,平均粒径为(245.7±1.3)nm,包封率为50.65%±1.36%,载药量为6.27%±0.29%,具有良好的缓释特性。体外实验中,纳米粒子能够被肿瘤细胞迅速摄取,Cur-RBC-NPs组有显著的抑制肿瘤生长效果。负载姜黄素的红细胞膜纳米粒子制备方法简便,具有良好的药物缓释性和抗肿瘤能力,有望成为新型的有临床应用前景的纳米给药系统。
The objective of this study is to develop a new-type biodegradable, biocompatible curcumin-loaded nanoerythrosomes （Cur-RBC-NPs） by means of the sonication method. The size of Cur-RBC-NPs was optimized by varying drug loading parameters. The morphology, size distribution, stability, in vitro release pattern, cellular uptake of nanoparticles and in vitro anti-tumor effects were evaluated, respectively. The results showed the prepared Cur-RBC-NPs were nearly uniform spheres, with an average diameter of （245.7 ±1.3） nm. Encapsulation efficiency （EE） and load efficiency （LE） of Cur-RBC-NPs were 50. 65% ±1.36% and 6. 27% ±0. 29%. And the nanoparticles had a good sustained release property. According to the in vitro experiment, Cur-RBC-NPs were effectively taken in by tumor cells, and exhibited a significant anti-tumor effect. In conclusion, the method for preparing Cur-RBC-NPs is convenient, with a good sustained release behavior and anti-tumor efficacy, and so expected to be a new-type nano-drng delivery system in clinical practice.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2016年第11期2093-2097,共5页
China Journal of Chinese Materia Medica
基金
国家自然科学青年基金项目(81502037)
江苏省自然科学青年基金项目(BK20150103)
关键词
姜黄素
红细胞
红细胞膜
纳米粒子
抗肿瘤
cummin
erythrocytes
erythrocyte membrane
nanoparticles
anti-tumor
