摘要
目的:本研究旨在探讨宁肾颗粒对脂多糖(LPS)诱导大鼠系膜细胞(MCs)增殖及凋亡的影响。方法:采用MTT法、细胞流式技术、Western bolt法检测不同剂量宁肾颗粒中药血清对LPS诱导大鼠系膜细胞增殖、凋亡及血小板源生长因子-BB(PDGF-BB)蛋白表达的作用。结果:含药血清能抑制LPS诱导大鼠MCs增殖、促进MCs凋亡,下调PDGF-BB蛋白的过度表达。结论:宁肾颗粒可抑制LPS诱导的MCs异常增殖、促进MCs凋亡,其分子机制可能与抑制PDGF-BB蛋白的过度表达有关。
This study aimed to evaluate the effects of Ningshen granule on the proliferation and apoptosis of glomerular mesangial cells（MCs） induced by lipopolysaccharide（LPS）, and the expression of platelet-derived growth factor-BB（PDGF-BB）. The drug-contained serum of model rats were prepared and used for intervention of LPS-stimulated glomerular MCs. The proliferation of LPS-stimulated MCs was measured by the MTT assay. Cell apoptosis rate was detected by flow cytometry, while the expression of PDGF-BB protein was semi-quantified by western bolt. It was found that the proliferation of MCs was inhibited by the Ningshen granule-contained serum, while the apoptosis of LPS-stimulated MCs. Besides, the overexpression of PDGF-BB was reversed. It was concluded that Ningshen granule inhibited the proliferation and promoted the apoptosis of LPS-stimulated MCs of rats, which may connect with the inhibition of overexpression of PDGF-BB.
出处
《世界科学技术-中医药现代化》
2016年第5期863-867,共5页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
山东省中医药管理局中医药科技发展计划课题(2007-124):宁肾颗粒对IgA肾病系膜细胞凋亡的影响及其机制
负责人:李安源
