摘要
目的 :探讨穹窿主体蛋白(major vault protein,MVP)对血管平滑肌细胞(vascular smooth muscle cells,VSMCs)增殖的影响。方法:分离培养野生型小鼠VSMCs,用血小板源性生长因子(PDGF-BB)或15%胎牛血清(FBS)刺激VSMCs增殖,Western blot检测VSMCs的MVP表达水平,同时检测特异性分化标记物α-SMA和SM22α表达水平。然后用PDGF-BB刺激人主动脉平滑肌细胞(HAo SMCs),检测MVP的表达差异。再分别分离培养野生型小鼠和MVP敲除型小鼠的VSMCs,通过CCK8细胞增殖实验检测两者的增殖能力;同时用si RNA干扰的方法,使HAo SMCs中MVP低表达,检测其增殖能力的变化。结果:用PDGFBB或15%FBS刺激VSMCs后,MVP表达水平呈浓度和时间依赖性下降,α-SMA和SM22α表达水平也相应降低。MVP缺失或低表达情况下,VSMCs的增殖能力均下降。结论:MVP在VSMCs增殖中起了重要作用。
Objective：To investigate the effects of major vault protein（MVP）on the proliferation of vascular smooth muscle cells（VSMCs）. Methods：The wild-type mouse VSMCs were isolated,and cultured,and the human aortic smooth muscle cells（HAo SMCs）were used. Western blot analysis was used to determine the MVP,α-SMA and SM22α. Expression of MVP stimulated by plateletderived growth factor（PDGF-BB）or 15% fetal bovine serum（FBS） was measured. MVP si RNA was used to downregulate the MVP expression in HAo SMCs. Cell proliferation CCK-8 assay was conducted to detect the proliferation ability of VSMCs in the case of MVP deficiency or downregulation. Results：After the treatment of PDGF-BB or 15% FBS,the MVP expression of VSMCs was downregulated in a time-dependent and dose-dependent manner. Compared with wild-type mouse VSMCs,the proliferation of MVP in knockout mouse VSMCs was suppressed. MVP si RNAs inhibited FBS-induced HAo VSMCs proliferation. Conclusion：MVP may played crucial effects in the proliferation of the VSMCs.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2016年第5期539-543,共5页
Journal of Nanjing Medical University(Natural Sciences)
基金
国家自然科学基金重点项目(81230070)
973项目(2012CB517503)
关键词
穹窿主体蛋白
小鼠主动脉平滑肌细胞
人主动脉平滑肌细胞
增殖
major vault protein
primary mouse aortic vascular smooth muscle cells
human aortic smooth muscle cells
proliferation
