摘要
目的：研究基因敲减配对相关同源框1 （prrxl）的表达对脐带间充质干细胞（UMSCs）增殖能力和多能性的影响.方法：胚胎干细胞-多能成体祖细胞-间充质干细胞（ESCs-MAPCs-MSCs）全基因组芯片筛选不同干细胞中差异表达的mRNA,针对所选基因特定序列构建prrx1-siRNA,转入UMSCs,real-time PCR验证干扰效率,检测增殖相关基因ki67,多能性相关基因oct4、sox2、nanog,上皮相关基因cdh1、epcam的表达,免疫荧光实验检测ki67蛋白表达.结果：干扰prrx1基因能明显促进增殖相关标志物ki67表达,促进多能性相关基因oct4、sox2、nanog的表达,促进上皮相关基因cdh1、epcam的表达,但不能使细胞发生形态上的改变.结论：下调MSCs中的相对高表达的prrx1基因表达,可促进UMSCs增殖能力与多能性.
Objective To investigate the effett of prrx1 knocked down on proliferation and pluripotency of mesenchymal stemcells（MSCs） . Methods ： Whole-genome mRNA microarryy analysis was used between ESCs,MAPCs,MSCs. P rrx1-siRNA was designed according to the varied gene prrx1. UMSCs were cultured and transfected by siRNA Real-time to confirm the interference efficiency and to detect the expression of proliferation related gene ki67, pluripotency related genes oct4,sox2, nanog, and epithelia related genes cdh1, epcam. The expression of proliferation related protein ki67 was detectedthrough immunofluorescence. Results： Prrx1 knockdown promoted the expression of proliferation marker ki67, pluripotentgenes oct4 , sox2, nanog and epithelia related genes cdh1, epcam. Butmorphologicll structure of UMS Csdidn’t change.Conclusion： Knocking down the MSCs-high expression gene prrx1 can promote the proliferation and pluripotency of UMSCs.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2016年第3期268-271,共4页
Chinese Journal of Anatomy
关键词
配对相关同源框1&脐带间充质干细胞
增殖
多能性
间充质上皮转化
paired related homeobox 1 ＆ umbilicll mesenchymll stem ce l ls
proliferation
pluripotency
mesenchym lepithelial transition
