摘要
建立了液相色谱-串联质谱法测定大鼠尿液和粪便中的银杏内酯B。以白果内酯为内标,采用电喷雾电离源负离子模式（ESI-）、多反应监测（MRM）进行定量分析。监测离子对m/z 423.1→m/z 367.1（银杏内酯B）和m/z 325.2→m/z163.2（白果内酯）。银杏内酯B在1-250μg/ml（尿液）和1.6-400.0μg/g（粪便）范围内线性关系良好,方法回收率为81.8%～113.8%（尿液）和83.5%-115.2%（粪便）,日内、日间RSD小于9.7%（尿液）和10.4%（粪便）。采用该法考察大鼠静脉注射银杏内酯B后的排泄动力学,48 h尿液与粪便合计累积排泄率为54.09%。
A LC-MS/MS method was established for the determination of ginkgolide B in rats urine and feces. The detection of the analyte and the internal standard （bilobalide） were conducted under negative ionization mode with an electrospray ionization （ESI） interface. A multiple reaction monitoring mode was chosen with the transitions of m/z 423.1→m/z 367.1 （ginkgolide B） and m/z 325.2→m/z 163.2 （bilobalide）. It was linear in the concentration range of 1--250 μg/ml （urine） and 1.6--400.0 μg/g（feces）. The method recoveries were 81.84%--113.8% （urine） and 83.50%--115.2% （feces）. The intra- and inter-day RSDs were less than 9.7% （urine） and 10.4% （feces）. The excretion pharmacokinetic in rats after intravenous injection of ginkgolide B was also investigated. The accumulated excretion rate of ginkgolide B in urine and feces was 54.09 % within 48 h.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2016年第6期738-742,共5页
Chinese Journal of Pharmaceuticals
