摘要
目的探讨右美托咪定对脓毒症大鼠肺组织高迁移率族蛋白B1(HMGB1)表达的影响。方法健康雄性SD大鼠45只,体重250-300g,采用随机数字表法,将大鼠随机分为3组(n=15):假手术组(SHAM组)、脓毒症模型组(CLP组)和右美托咪定+脓毒症组(DEX组)。CLP组和DEX组采用盲肠结扎穿孔法(CLP)建立脓毒症模型。DEX组于术后1h腹腔内注射盐酸右美托咪定溶液40μg/kg,SHAM组和CLP组分别给予等容量生理盐水。分别于术后6h、24h、48h时各组随机处死5只大鼠取肺组织,采用逆转录聚合酶链式反应(RT-PCR)技术检测HMGB1 m RNA的表达,采用蛋白免疫印迹法(Western blotting)测定肺组织HMGB1蛋白的含量,苏木精-伊红染色观察肺组织的病理学形态变化。结果与SHAM组比较,CLP组和DEX组各时点大鼠肺组织HMGB1 m RNA和蛋白表达水平均上调;与CLP组比较,DEX组大鼠肺组织HMGB1 m RNA和蛋白表达水平均下调。DEX组肺组织病理学损伤较CLP组减轻。结论右美托咪啶可显著减轻脓毒症大鼠肺损伤,其机制可能与其抑制HMGB1表达上调有关。
Objective To investigate the effect of dexmedetomidine on expression of high mobility group box 1（HMGB1） m RNA in lung tissue in septic rats. Methods 45 male Sprague Dawley rats weighing 250-300 g were randomly divided into 3 groups（n =15）：Sham operation group（group Sham）,Sepsis group（group CLP）、Dexmedetomidine group（group DEX）. Each rat had cecal ligation and puncture surgery（CLP） to establish model of sepsis except those in group Sham. Group DEX was given intraperitoneally dexmedetomidine in a dose of 40μg/kg at 1 hour after CLP. Isometric normal saline was given intraperitoneally in group Sham and group Clp. The lung tissues were taken at 6,24 and 48 hour after CLP. The expression of the HMGB1 m RNA were examined by RT-PCR and the level of HMGB1 protein in the lungs were detected by Western blot. The acute lung injury was detected by HE stain. Results Compared with group Sham,the expression of the HMGB1 m RNA and the level of HMGB1 protein were increased in group Clp and group Dex. Compared with group Clp,the expression of the HMGB1 m RNA and the level of HMGB1 protein were decreased in group Dex. The injury of pathology of lung was slighter in group Dex than in group Clp. Conclusion Dexmedetomidine can reduce lung injury through inhibiting HMGB1 expression in septic rats.
出处
《江西医药》
CAS
2016年第5期415-417,423,共4页
Jiangxi Medical Journal
