摘要
目的观察加减薯蓣丸对血管性痴呆模型大鼠行为学及海马区神经营养因子3(NT-3)表达的影响,探讨其改善学习记忆的可能机制。方法选用SPF级雄性Wistar大鼠60只,随机选取其中20只作为假手术组,其余大鼠采用双侧颈总动脉分次结扎并剪断的方法制作血管性痴呆模型。将造模成功大鼠40只随机分为模型组和中药组各20只,中药组给予0.01 m L/g加减薯蓣丸灌胃,其余2组灌胃等量生理盐水,均1次/d。灌胃8周后进行Morris试验,Morris试验结束后取海马组织采用荧光PCR法检测海马区NT-3表达水平。结果与假手术组比较,模型组大鼠逃避潜伏期显著延长,穿越平台次数减少,平台象限停留时间缩短,大鼠海马区NT-3表达明显下降,差异均有统计学意义(P均<0.05);与模型组比较,中药组大鼠逃避潜伏期明显缩短,穿越平台次数增加,平台象限停留时间延长,大鼠海马区NT-3表达显著升高,差异均有统计学意义(P均<0.05)。结论加减薯蓣丸可能通过提高海马区NT-3表达水平,影响神经突触可塑性,从而发挥对抗血管性痴呆的作用。
Objective It is to observe the influence of Modified Yam Pill（ MYP） on the expression of Neurotropin- 3（ NT-3） in the hippocampus of vascular dementia rats,and explore the possible mechanism of improving learning and memory.Methods 60 cases of male Wistar rats in SPF grade were selected,20 cases of them were randomly selected as sham operation group,and the others were treated by bilateral common carotid artery ligation and cut to make vascular dementia model. 40 cases of successful rat model were randomly divided into model group and Chinese medicine group with 20 cases in each groups,the animals were given 0. 01 m L / g MYP to fill the stomach in Chinese medicine group,and the other two groups were given the same amount of normal saline,1 times per day. Morris test was carried out after 8 weeks of filling the stomach,the hippocampal tissue was taken to detect the expression level of NT- 3 using fluorescence PCR method after the Morris test. Results Compared with sham operation group,the escape latency was significantly longer,and the number of cross platform was reduced,and the platform quadrant dwell time was shorter,and the expression of NT- 3 in hippocampus was decreased significantly in the model group,there were significant difference（ all P 0. 05）. Compared with model group,the escape latency was significantly shorten,and the number of cross platform was increase,and the platform quadrant dwell time was longer,and the expression of NT- 3 in hippocampus was increased in the Chinese medicine group,there were significant difference（ all P 0. 05）. Conclusion MYP can enhance the expression of NT- 3 in the hippocampus,and affect the plasticity of the synapse,so as to play a role in anti vascular dementia.
出处
《现代中西医结合杂志》
CAS
2016年第18期1939-1941,1956,共4页
Modern Journal of Integrated Traditional Chinese and Western Medicine
基金
湖北省科技厅自然基金资助项目(2010CDB11002)
