摘要
目的:比较补阳还五汤含药血清和含药血浆对缺氧培养的大鼠脑微血管内皮细胞(BMECs)分泌NO、v WF、6-ketoPGF1α的水平与体内实验真实值的接近程度,为半体内实验选择含药血浆还是含药血清提供参考依据。方法:以氧剥夺BMECs模型为研究对象,观察补阳还五汤含药血浆和含药血清对BMECs条件培养液中一氧化氮(NO)、血管性假血友病因子(v WF)、6-酮-前列腺素1α(6-keto-PGF1α)含量的影响,并与补阳还五汤干预的中动脉阻塞模型大鼠血浆上述指标水平进行比较。结果:结果发现:与空白血浆组合空白血清组比较,补阳还五汤含药血浆和含药血清均能明显降低氧剥夺BMECs条件培养液中v WF的含量,升高NO、6-keto-PGF1α水平,差异均有统计学意义(P<0.05或P<0.01);含药血浆组v WF、6-keto-PGF1α水平与体内实验的相关系数r分别为0.928和0.933,明显高于含药血清组(r=0.715、0.770,P<0.05),而含药血浆组和含药血清组NO含量与体内实验相关系数分别为0.702、0.759,两者差异无统计学意义(P>0.05)。结论:运用半体内实验研究补阳还五汤对脑血管内皮细胞的作用,血浆药理学方法比血清药理学方法可靠。
Objective： To compare the approaching degree of the expression of NO,v WF and 6-keto-PGF1 a between Yang-Tonifying Five-Returning Decoction（ YFD） contained plasma and serum on brain microvascular endothelial cells（ BMECs） injured by hypoxia rats with true value of animals in vivo experiments,and to provide references for selecting plasma or serum in the study of ex vivo experiments. Methods： The model of MCAO was made by Suture Embolization Method in the internal carotid artery of rats.BMECs were made in hypoxia incubator. The method of Enzyme-linked immunosorbent（ ELISA） was used to measure the expression of NO,v WF and 6-keto-PGF1 a. Results： Both of medicated plasma and medicated serum of YFD could significantly reduce the content of v WF,increase the expression of NO and 6-keto-PGF1 a. There were significant differences（ P〈0. 05 or P〈0. 01）compared with blank plasma and blank serum; The correlation coefficients of v WF between medicated plasma and in vivo experiments was higher than those between medicated serum and in vivo experiments. There was significant difference. The correlation coefficients of NO and 6-keto-PGF1 a between medicated plasma and in vivo experiments were in close proximity to those between medicated serum and in vivo experiments. There were no significant differences. Conclusion： When studying the expression of v WF in the ex vivo experiment,it is suggested that the plasma pharmacology method should be used. When studying the expression of NO and 6-keto-PGF1 a,the methods of plasma pharmacology and serum pharmacology can be both selected.
出处
《世界中医药》
CAS
2016年第4期604-608,共5页
World Chinese Medicine
基金
国家自然科学基金项目(编号:81274008)
湖南省科研条件专项课题(编号:2011TT2050)
湖南省科技厅一般项目(编号:2014SK3052)
