摘要
为了探究光敏性碳基纳米材料的生物富集过程,通过一步微波热解反应合成14 C标记的碳点,并以海洋原甲藻(Prorocentrum micans)和东海原甲藻(Prorocentrum donghaiense)作为受试生物,定量分析了这两种生物对碳点(C-dots)的吸收动力学差异.由于生物细胞的遮挡效应可能会影响胞内标记碳点的测定,实验中首先比较了生物样品三种处理方法(对生物样品分别直接测定,超清洗,超声破碎)的效果差异,发现对生物细胞样品进行超声破碎的处理效果最好,并且在超声5min的条件下测定效果最佳.随后,开展实验进一步探究了短时间内碳点在生物细胞内的富集过程.结果表明,碳点在这两种生物体内的富集量都随时间呈现线性增加的趋势.当我们以三种生物富集量表示方法作图(pg·cell-1,pg·μm-2和pg·μm-3)时,发现采用以单位体积的生物富集量作图最科学,并且发现这两种原甲藻对碳点的生物富集量随时间的变化趋势极为接近,克服了藻细胞大小带来的差异,更为科学地阐述了水生生物体内碳点的富集量的变化.
To investigate the bioaccumulation of carbon-based nanomaterial,we synthesized the14 C-labeled carbon dots（C-dots）by one-way microwave pyrolysis.Two marine algae,Prorocentrum micans and Prorocentrum donghaiense were used as the objective organisms,and their accumulation of C-dots was compared.As the 14 C signal from the Cdots may be quenched inside the cells,the results of the different ultrasonicating methods were compared,and the counting number was the highest when the cells were ultrasonicated for 5minutes.After the optimization of the cell processing methods,we further explored the bioaccumulation kinetics of C-dots by both algae and the accumulationresults with different units（pg·cell-1,pg·μm-2,and pg·μm-3）were compared.As expected,cellular concentration of C-dots increased linearly with exposure time regardless of the units of the accumulation results.Nevertheless,when the accumulation results were expressed in the unit of pg·μm-3,potential accumulation difference between the two algae disappeared and this unit would be used in the bioaccumulation studies of C-dots in the future.
出处
《南京大学学报（自然科学版）》
CAS
CSCD
北大核心
2016年第3期512-519,共8页
Journal of Nanjing University（Natural Science）
基金
海洋公益性行业科研专项(201505034)
国家自然科学基金(41271486,41001338,21237001)
关键词
碳基纳米材料
碳点
东海原甲藻
海洋原甲藻
超声破碎
生物富集
carbon-based nanomaterials
carbon dots
Prorocentrum donghaiense
Prorocentrum micans
ultrasonication
bioaccumulation
