摘要
目的分析临床分离表皮葡萄球菌对不同类型氨基糖苷类抗菌药物的耐药特征;分析icaADBC操纵子在临床分离的表皮葡萄球菌中的检出及与细菌耐药的关系。方法收集2013年1~10月上海市东方医院临床分离的表皮葡萄球菌77株,采用纸片扩散法检测细菌4种氨基糖苷类抗菌药物（庆大霉素、链霉素、奈替米星和阿米卡星）药敏试验特征;构建icaADBC特异性引物,采用聚合酶链反应扩增技术检测77株细菌中icaADBC的分布状况。结果结果77株表皮葡萄球菌对氨基糖苷类抗菌药物耐药率较高,分别为庆大霉素：62.3%,链霉素：61.0%,阿米卡星：22.1%,奈替米星：23.4%。icaADBC操纵子的检出率为20.8%,ica阳性菌株与阴性菌株对氨基糖苷类抗菌药物耐药性比较差异有统计学意义（P〈0.05）。结论临床分离的icaADBC操纵子阳性表皮葡萄球菌对氨基糖苷类抗菌药物耐药率较高。
Objective To analyze the antimicrobial susceptibility of Staphylococcus epidermidis to different kinds of aminoglycoside and to explore the relationship between icaADBC in Staphylococcus epidermidis and the resistance to aminoglycosides.Methods Antimicrobial susceptibility to different aminoglycosides including gentamicin,streptomycin,netimicin and amikacin were tested in 77 isolated strains by the agar diffusion method.The iac ADBC specific primers were constructed.And the icaADBC of 77 strains were detected by PCR amplification technology.Results The resistance to aminoglycosides were as following：23.4%,61.0%,22.1% and 62.3%of the 77 strains were resistant to netilmicin,streptomycin,amikacin and gentamicin,respectively.Among the 77 stains,the positive rate of icaADBC was 20.8%.The differences of resistance to aminoglycosides between ica positive strains and ica negative strains had statistic significance（P〈0.05）.Conclusion Clinical Staphylococcus epidermidis isolated with icaADBC show high percentage of resistance to aminoglycosides.
出处
《国际检验医学杂志》
CAS
2016年第11期1479-1481,共3页
International Journal of Laboratory Medicine
