摘要
目的 探讨应激对重性抑郁障碍（major depressive disorder,MDD）患者脑源性神经营养因子（Brain-derived neurotrophic factor,BDNF）基因甲基化影响,及BDNF基因甲基化和MDD的关系.方法 采用病例-对照研究方法,选取47例MDD患者分为应激组（24例）、非应激组（23例）与27例正常对照作为研究对象.采用重亚硫酸氢盐测序法检测外周血BDNF基因启动子区CpG岛甲基化状态.运用SPSS17.0统计软件包进行数据处理.分析MDD应激组、非应激组及正常对照组19个CpG位点甲基化差异及三组甲基化整体水平.结果 MDD应激组、非应激组与正常对照组CpG整体甲基化率（中位数,四分位数间距）分别为189.150（7.575）,188.500（4.000）,480.200（7.700）,差异有统计学意义（P＜0.01）;MDD应激组与非应激组CpG整体甲基化水平比较,差异无统计学意义（P＞0.05）.MDD应激组、非应激组与正常对照组CpG位点甲基化率（均数±标准差或中位数,四分位数间距）分别为CpG-1：2.600（0.275）,2.700（0.400）,6.500（0.800）;CpG-2：3.350（0.650）,3.300（0.800）,14.600（1.500）;CpG-3：（1.596±0.363）,（1.543±0.400）,（4.581±0.437）;CpG-4：（1.779±0.516）,（1.522±0.329）,（4.033±0.529）;CpG-5：0.900（0.575）,0.800（0.600）,5.700（1.500）;CpG-6：（6.258±0.805）,（6.213±0.944）,（14.589±0.819）;CpG-7：（10.667±0.894）,（10.283± 1.006）,（15.000±0.763）;CpG-8：（16.421±0.697）,（16.330±0.775）,（24.796±0.547）;CpG-9：（4.713±0.565）,（4.891 ±0.554）,（28.826±0.679）;CpG-10：（10.254±0.902）,（10.378±0.777）,（11.381 ±0.538）;CpG-11：（24.125±2.301）,（24.170±2.613）,（37.474± 1.579）;CpG-12：（5.442±0.641）,（5.596± 1.117）,（12.141 ±0.940）;CpG-13：4.150（1.150）,4.200（1.000）,61.700（4.800）;CpG-14：（5.500±0.544）,（5.717±0.568）,（6.378±0.397）;CpG-15：3.700（0.700）,4.100（1.000）,63.300（2.500）;CpG-16：8.200（1.775
Objective To investigate the effects of stress on the methylation of brain derived neurotrophic factor gene in the patients with major depressive disorder （MDD）,and to investigate the relationship between BDNF gene methylation and MDD.Methods 47 cases of MDD were divided into MDD stress group （n=24） and MDD non stress group（n=23） while 27 health subjects were collected as normal control group.The methylation status of CpG island in the promoter region of BDNF gene in peripheral blood was detected by the method of heavy hydrogen and hydrogen sulfate.SPSS17.0 statistical software package was used to analyze the data.The differences of 19 CpG methylation rates and the overall level of methylation rates of three groups were analyzed.Results The CpG overall methylation rates （median,interquartile range） of MDD stress group,MDD non stress group and normal control group was 189.150 （7.575）,188.500 （400）and 480.200（770） respectively,and the difference was statistically significant （P〈0.01）.There was no significant difference in the overall methylation rate of CpG in the MDD stress group compared with MDD non stress group （P〉0.05）.The CpG methylation rates （mean± SD or median,interquartile range） of three groups were detected as follows：CpG-1：2.600（0.275）,2.700 （0.400）,6.500（0.800）;CpG-2：3.350（0.650）,3.300（0.800）,14.600（1.500）;CpG-3：1.596±0.363,1.543±0.400,4.581 ±0.437;CpG-4：1.779±0.516,1.522±0.329,4.033 ±0.529;CpG-5：0.900 （0.575）,0.800 （0.600）,5.700 （1.500）;CpG-6：6.258 ± 0.805,6.213 ±0.944,14.589±0.819;CpG-7：10.667±0.894,10.283± 1.006,15.000±0.763;CpG-8：16.421 ±0.697,16.330±0.775,24.796±0.547;CpG-9：4.713±0.565,4.891 ±0.554,28.826±0.679;CpG-10：10.254±0.902,10.378±0.777,11.381±0.538;CpG-11：24.125±2.301,24.170±2.613,37.474± 1.579;CpG-12：5.442±0.641,5.596±1.117,12.141 ±0.940;CpG-13：4.150（1.150）,4.200（1.000）,61.700（4.800）;CpG-14：5.500±0.544,5.717±0.568,6.378±0.397;CpG-15：3.
出处
《中华行为医学与脑科学杂志》
CAS
CSCD
北大核心
2016年第5期437-441,共5页
Chinese Journal of Behavioral Medicine and Brain Science
关键词
重性抑郁障碍
脑源性神经营养因子
应激
甲基化
Major depressive disorder
Brain-derived neurotrophic factor
Stress
Gene methylation
