摘要
目的研究地椒抗氧化活性成分,阐明地椒抗氧化活性化学成分组成。方法采用1,1-diphenyl-2-picrylhydrazyl（DPPH）和2,2＇-azino-bis（3-ethylbenzothiazoline-6-sulphonic acid）（ABTS）自由基清除试验筛选评价地椒5个极性部位的抗氧化活性;采用硅胶、Sephadex LH-20和MCI GEL CHP-20P等柱色谱方法进行分离纯化,并根据质谱、核磁数据和参考文献数据等鉴定化合物的结构。结果地椒乙酸乙酯层和正丁醇层显示很好的抗氧化活性。从该两萃取层中,分离鉴定18个化合物分别为野黄芩苷（1）,野黄芩素（2）,5,6,7-三羟基-4＇-甲氧基黄酮（3）,4＇5-二羟基-6,7,8,-三甲氧基黄铜（xanthomicrol）（4）,芹菜素（5）,4＇-甲氧基木犀草素（6）,木犀草苷（7）,木犀草素（8）,芦丁（9）,丹参素（10）,香草酸（11）,原儿茶酸（12）,绿原酸（13）,咖啡酸（14）,阿魏酸（15）,2,6-二羟基-4-异丙基-β-D-葡萄糖苷（16）,齐墩果酸（17）和胡萝卜苷（18）。结论化合物1~3和16首次从百里香属植物中分离得到,化合物4~8,10~12,14~15和17~18首次从地椒植物中分离得到。
Objective To study the antioxidant activities and chemical compositions of Thymus quinquecostatus Celak（ dijiao）. Methods The antioxidant activities of five polar fractions of dijiao were screened and evaluated using DPPH and ABTS free radical scavenging assays. After the chemical compositions were separated and purified by using silica gel chromatography,Sephadex LH-20 chromatography and MCI GEL CHP-20 P column chromatography,the chemical compositions were identified by MS and NMR spectrometers,and records of literature. Results From the Et OAc and n-Bu OH fractions which showed high antioxidant activity,total 18 compositions were separated and identified： scutellarin（ ＂1 ＂short for peak No. 1,the same below）,scutellarein（ 2）,5,6,7-trihydroxy-4＇-methoxyflavone（ 3）,xanthomicrol（ 4）,apigenin（ 5）,4＇-methoxyluteolin（ 6）,luteolin-7-O-β-D-glucopyranoside（ 7）,luteolin（ 8）,and rutin（ 9）,danshensu（ 10）,vanillic acid（ 11）,protocatechuic acid（ 12）,chlorogenic acid（ 13）,caffeic acid（ 14）,ferulic acid（ 15）,2,6-dihydroxy-4-isopropylphenyl-β-D-glucopyranoside（ 16）,olenolic cid（ 17） and daucosterol（ 18）. Conclusion The composition 1,2,3 and 16 were the first reported in the genus Thymus,and 4 ~ 8,10 ~ 12,14 ~ 15 and 17 ~ 18 were found first in dijiao.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2016年第5期383-389,共7页
Journal of Beijing University of Traditional Chinese Medicine
基金
北京中医药大学创新团队项目(No.2011-CXTD-12)
北京中医药大学自主课题项目(No.2014-JYBZZ-JS-026)
陕西省农业公关项目(No.S2014NY2608)
