摘要
目的探讨汽油中挥发性有机物(volatile organic matters,VOCs)对肺组织的损伤作用。方法将SPF级雄性昆明小鼠40只随机分为对照组和汽油低、中、高剂量组,每组10只。分别称取汽油1、2、4 g置于静式染毒柜中作为染毒的低、中、高剂量,待挥发30 min后将小鼠置于染毒柜中,每天染毒2 h,持续28 d。减重法测得低、中、高剂量组染毒柜内的VOCs浓度分别为9.524、18.873、34.746 mg/m^3。用试剂盒检测超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力及丙二醛(MDA)含量,并观察肺组织形态学变化。结果汽油高剂量组小鼠的肺组织MDA含量高于对照组,SOD和GSH-Px活力低于对照组,差异均有统计学意义(P<0.05)。各染毒组小鼠肺组织均可见肺泡壁增生变厚、炎细胞浸润。结论汽油VOCs可引起小鼠肺组织氧化损伤和病理学改变。
Objective To investigate the damage effect of volatile organic compounds（VOCs） on the lungs of mice. Methods A total of 40 clean grade Kunming mice were randomly divided into four groups, 10 in each, control group, low dose group of gasoline（1 g）, moderate dose group of gasoline（2 g） and high dose group of gasoline（4 g）. The static type exposure chamber was used and mice were exposed to gasoline after volatilization for half an hour. The exposure time was 2 hours every day for 28 days. The concentration of VOCs in low dose group, moderate dose group and high dose group detected by weight loss method were 9.524, 18.873 and 34.746 mg/m^3 respectively. After the experiment, the levels of SOD, GSH-Px and MDA were determined, and the lung tissue changes were examined. Results Compared with the control group, MDA increased, GSH-Px and SOD decreased significantly in the lung tissue in high dose group（P〈0.05）. The alveolar walls were thickening with inflammatory cells infiltrated. Conclusion Volatile organic compounds of gasoline may cause lung injury in mice.
出处
《环境与健康杂志》
CAS
北大核心
2016年第3期208-209,282,共2页
Journal of Environment and Health
基金
国家环保部科研专项(201209003)
