摘要
目的:考察超声波辅助乙醇提取法对东北茶藨子叶片中总酚、总黄酮提取含量的影响。方法:采用比色法测定总酚、总黄酮的含量。在单因素实验的基础上,通过Box-Behnken实验设计(BBD)结合响应面法(RSA)优化提取条件,并对不同提取方法进行了比较。结果:超声波法提取东北茶藨子叶片中总酚(以没食子酸计)、总黄酮(以芦丁计)的最佳提取条件为乙醇浓度70%、液固比24 m L/g、超声时间30 min、超声温度40℃、超声功率200 W,在此条件下东北茶藨子叶片中总酚、总黄酮含量分别为135.80 mg/g和13.56 mg/g。结论:超声辅助乙醇提取法高效、快速,可以作为东北茶藨子叶片中总酚、总黄酮的提取方法。
Objective：Ethanol ultrasonic-assisted extraction of total phenols and flavonoids from Ribes mandshuricum（Maxim.）Kom leaves was investigated to optimumize the extraction conditions.Method：Colorimetric method was used to determine the total content of phenols and flavonoids.Based on the single factor,we used Box-Behnken Design combined with response surface analysis to optimize extraction conditions,finally compared the different extracting methods.Results：The optimum condition of extracting total phenols（Gallic acid/g extract） and flavonoids（rutin/g extract） was as follows：70% ethanol,ratio of liquid to material of 24 m L/g,at40℃,under 200 W,for 30 min.The yields of total phenols and flavonoids could reach 135.80mg/g and 14.56mg/g,respectively.Conclusion：With its high efficiency as well as rapid extracting speed,Ultrasound-assisted alcohol extraction method is suitable to extract total phenols and flavonoids from Ribes mandshuricum（Maxim.）Kom leaves.
出处
《黑龙江医药》
CAS
2016年第2期234-240,共7页
Heilongjiang Medicine journal
基金
E类中央高校基本科研业务费专项资金项目(创新团队与重大项目培育资金项目)-茶藨子属植物花发育及次生代谢产物合成分子调控机制研究(2572014EA03)
黑龙江省博士后科研启动金:茶藨子属植物花发育及次生代谢产物合成分子调控机制研究(LBH-Q14011)
关键词
超声辅助
东北茶藨子叶片
总酚
总黄酮
响应面分析法
ultrasound-assisted
the leaf of Ribes mandshuricum
total phenols
total flavonoids
response surface analysis
