摘要
目的 探讨5,10-亚甲基四氢叶酸还原酶（MTHFR）基因C677T位点多态性与不明原因胎停育的关系.方法 采用基因芯片法分析MTHFR基因C677T位点多态性;选取2014年8月至2015年9月在周口市中心医院不孕不育科就诊的原因不明胎停育≥2次的349例孕妇为病例组,421例无流产史健康经产妇为对照组.比较病例组与对照组基因型、等位基因频率分布,分析胎停育与MTHFR C677T位点多态性的关系.结果 两组MTHFR C677T基因型C/C分布差异有统计学意义[P ＜0.001、χ^2=70.484、OR=0.267、95％ CI （0.195-0.366）];两组基因型C/T分布差异无统计学意义[P =0.714、χ^2=0.156、OR=1.06、95％ CI （0.795-1.412）];两组基因型T/T分布差异有统计学意义[P ＜0.001、χ^2=98.812、OR =7.961、95％ CI （5.055- 12.537）];两组等位基因C分布差异有统计学意义[P ＜0.001、χ^2=132.87、OR =0.291、95％ CI（0.236-0.361）],两组等位基因T分布差异有统计学意义[P ＜0.001、χ^2=132.87、OR =3.431、95％ CI （2.772 -4.246）].结论 育龄女性不明原因胎停育可能与MTHFR基因C677T位点突变有关,T/T基因型高表达可能为其危险因素.
Objective To investigate the 5,10-methylenetetrahydrofolate reductase （MTHFR） gene C677T polymorphism and unexplained embryo arrest relationship.Methods By the method of gene chip analysis of MTHFR gene C677T polymorphism;select August 2014 and September 2015 in Zhoukou City Center Hospital infertility clinic unexplained Embryo arrest was more than or equal to 2 349 times pregnant women as the case group,and 421 without abortion history of health by women as the control group.The genotype and allele frequency distribution of embryo arrest and C677T MTHFR were compared between the experimental and control groups.Results MTHFR C677T genotype C/C in experimental and control groups were distributed with significant difference [P 〈 0.001,χ^2 =70.484,OR =0.267,95% CI （0.195 - 0.366）].Genotype C/T in experimental and control groups was distributed without significant difference [P =0.714,χ^2 =0.156,OR =1.06,95% CI （0.795 - 1.412）].T/T genotype in experimental and control groups was distributed with significant difference [P 〈 0.001,χ^2 =98.812,OR =7.961,95% CI （5.055 - 12.537）].C allele in experimental and control groups was distributed with significant difference [P 〈0.001,χ^2 =13.287,OR =0.291,95% CI （0.236 -0.361）].Allele t in experimental and control group was distributed with significant difference [P 〈0.001,χ^2 =13.287,OR =3.431,95% CI （2.772 -4.246）].Conclusions Embryo arrest was not associated with C677T gene mutations in MTHFR gene,and the high expression of T/T gene might be a risk factor for women of childbearing age.
出处
《中国医师杂志》
CAS
2016年第5期702-705,共4页
Journal of Chinese Physician
