摘要
目的探讨迷走神经刺激对兔脊髓损伤(SCI)后脊髓组织中肿瘤坏死因子-α(TNF-α)水平的影响,并与甲基泼尼松龙(MP)进行比较。方法 125只新西兰大白兔分为假手术组(SHAM组,25只)、SCI组(25只)、迷走神经刺激组(STM组,25只)、MP组(25只)和STM+MP组(25只)。采用改良Allen打击法制作兔脊髓损伤模型,STM组及STM+MP组于脊髓损伤后接受右侧颈迷走神经电刺激(5V、2ms和1Hz,持续20min)。术后1、4、8、12、24h分别采用酶联免疫吸附试验(ELISA)法及逆转录PCR(RT-PCR)法检测各组脊髓组织中TNF-α蛋白水平及mRNA水平。结果与SCI组相比,STM组、MP组、STM+MP组的脊髓组织中TNF-α蛋白水平均有明显下降,其中STM+MP组降低最为显著;与SCI组和STM组相比较,在各个时间点MP组和STM+MP组的TNF-αmRNA水平下降明显。结论迷走神经刺激可降低兔急性脊髓损伤的促炎因子TNF-α蛋白水平,其效果接近于MP,且在抑制TNF-α蛋白表达方面与MP具有协同作用。
Objective To investigate the effects of vagus nerve stimulation on tumor necrosis factor-αexpression in spinal cord tissue after acute spinal cord injury（SCI）in rabbits.And a contrastive study was performed between vagus nerve stimulation and methylprednisolone（MP）.Methods A total of 125 New Zealand white rabbits were divided into sham-operated group（SHAM group,n=5）,SCI group（n=25）,vagus nerve stimulation group（STM group,n=25）,MP group（n=25）and STM＋MP group（n=25）.The SCI models of rabbits were established by the modified Allen′s weight hit.The right cervical vagus nerves of the rabbits in STM group and STM＋MP group were stimulated（5V,1 Hz,2ms,lasting 20min）.At the 1,4,8,12,24 hour after SCI,TNF-αprotein expressions of spinal cord tissue were detected through enzyme linked immuno sorbent assay（ELISA）and the mRNA levels of TNF-αwere measured by reverse transcription-polymerase chain reaction（RT-PCR）.Results The expressions of TNF-αprotein in the spinal cord of STM group,MP group and STM＋MP group were less than that of SCI group,and the greatest decrease was observed in STM＋MP group.Compared with SCI group and STM group,the expressions of TNF-αmRNA decreased significantly in both MP group and STM＋MP group at every time point.Conclusion STM could reduce the expressions of TNF-αin the spinal cord of rabbits after acute SCI.The effect of vagus nerve stimulation restrained the TNF-αexpressions is comparable to MP and have synergistic action with MP in terms of the inhibition of TNF-αexpressions.
出处
《重庆医学》
CAS
北大核心
2016年第15期2051-2054,共4页
Chongqing medicine
基金
安徽省教育厅高等学校省级自然科学研究项目(KJ2013Z149)
