摘要
目的:评估"菌bye-bye"消毒剂对甲型H1N1流感病毒PR8(PR8)、呼吸道合胞病毒(RSV)、3型腺病毒(ADV3)、人感染H7N9禽流感病毒(H7N9)的体外杀灭效果。方法:参考《消毒技术规范》和受试产品说明书进行细胞毒性试验、消毒剂中和试验和对PR8、RSV、ADV3、H7N9病毒的体外杀灭实验。结果:(1)候选中和剂选定0.02mol/L硫代硫酸钠(Na_2S_2O_4);(2)候选中和剂、"菌-bye-bye"消毒剂及两者混合物对细胞毒性低;(3)候选中和剂能完全中和消毒剂消毒效果;(4)随着"菌bye-bye"消毒剂的浓度增大,病毒杀灭效果亦越好。在其不稀释的条件下,除了PR8毒株外,其他3种病毒的平均灭活率均在99.99%以上,且平均病毒TCID50负对数值(log_(10)TCID_(50)/m L)均降低4 log_(10)或以上;对半稀释后对RSV的杀灭效果最好,而对ADV3的杀灭效果最差。结论:高浓度"菌bye-bye"消毒剂对呼吸道病毒的杀灭效果较好,且对细胞的毒性较小,是一种安全、高效的消毒剂,值得临床推广。
Objective： To evaluate the effect of ＂Bacteria-bye-bye＂disinfectant on killing H1N1 influenza virus PR8 （PR8）, respiratory syncytial virus （RSV）, adenovirus 3 （ADV3）, human H7N9 avian influenza （H7N9） in vitro. Methods： To performed the cytotoxicity test, disinfectant neutralization test and PR8, RSV, ADV3, H7N9 virus killing test in vitro according to the 〈Disinfection Technology Criteria〉and test product specification. Results： （1）The candidate neutralizing agent was 0.02 mol/L sodium hyposulfite （Na2S2O4）; （2）The cytotoxicity of candidate neutralizing agent, ＂Bacteria -bye-bye＂disinfectant and their mixture were low; （3）The candidate neutralizing agent could completely neutralize the effect of disinfectant; （4）The higher concentration of ＂Bacteria-bye-bye＂ disinfectant, the better effect on virus killing.In the undiluted condition, except PR8, the virus inactivation rate of other 3 viruses were above 99.99%, and the average viral TCID50 negative logarithm（log10 TCID50/mL） decreased more than 4 log10; After half to half diluted, it had the best killing effect on RSV, but the worst killing effect on ADV3. Conclusion： High concentration ＂Bacteria-bye-bye＂ disinfectant is effective on killing respiratory tract viruses, and has less toxic to cells,it is safe and high-performance, which is worthy of clinical promotion.
出处
《现代生物医学进展》
CAS
2016年第16期3105-3107,3157,共4页
Progress in Modern Biomedicine
基金
2014年度广东省医学科研基金项目(A2014295)
广东省省级科技计划(2013B020224006)
广州市科技计划项目-科技惠民专项(2014Y2-00031)
关键词
二氧化氯
消毒剂
呼吸道
病毒
感染
Chlorine dioxide
Disinfectant
Respiratory tract
Virus
Infection
