摘要
目的探讨生物钟(clock circadian regulator,CLOCK)基因与男性儿童注意缺陷多动障碍(atten-tion-deficit/hyperactivity disorder,ADHD)的关联,以及CLOCK基因与睡眠问题及其交互作用对ADHD男性患儿注意抑制的影响。方法对854例ADHD男性患儿与320名男性对照儿童的CLOCK基因rs6832769与rs11932595单核苷酸多态性位点(single nucleotide polymorphisms,SNPs)进行基因型检测。采用Conners父母症状问卷评估ADHD组与对照组的睡眠问题,通过Stroop色词命名测验评估ADHD组的注意抑制功能,通过协方差分析分别检验CLOCK基因2个SNPs和睡眠问题及其交互作用对ADHD男性患儿注意抑制的影响。结果rs6832769、rs11932595的等位基因频率、基因型频率与ADHD的关联均无统计学意义(P>0.05)。rs6832769、rs11932595基因型及睡眠问题对男性ADHD患儿注意抑制的主效应均没有统计学意义(P>0.05);rs6832769基因型与睡眠问题对注意抑制存在交互作用(F=6.71,P=0.01),伴睡眠问题且携带rs6832769位点AG&GG基因型患者的字义干扰时更长(F=6.63,P=0.01)。结论 CLOCK基因rs6832769与睡眠问题可能存在交互作用,共同影响ADHD男性患儿的注意抑制功能。
Objective To explore the correlation between circadian clock gene clock circadian regulator（CLOCK）and attention-deficit/hyperactivity disorder（ADHD） and the role of CLOCK and sleep problems on inhibition in malechildren with ADHD.MethodsTwo single nucleotide polymorphisms（SNPs） of CLOCK were genotyped in 854 maleADHD children and 320 male controls. Sleep problems were assessed using parent symptom questionnaire. In ADHDcases, the main effects and interaction of CLOCK SNPs and sleep problems on inhibition assessed by using Stroop Colorand Word Test, were analyzed using the analysis of covariance（ANCOVA）.ResultsNo significant differences of alleleand genotype frequencies were found for rs6832769 and rs11932595 in all case-control groups（P〉0.05）. In ADHD cas-es, the main effects of rs6832769 and rs11932595 genotypes and sleep problems on inhibition were not significant（P〉0.05）. However, the interaction of rs6832769 genotype and sleep problems was significant（F=6.71, P=0.01）. When ac-companied with sleep problems, ADHD cases carrying the AAAG genotype showed the longest time of word interfer-ence（F=6.63, P=0.01）.ConclusionsInhibition of male ADHD children can be modulated by the interaction of CLOCK rs6832769 and sleep problems.
出处
《中国神经精神疾病杂志》
CAS
CSCD
北大核心
2016年第4期193-198,共6页
Chinese Journal of Nervous and Mental Diseases
基金
国家重点基础研究发展计划(973计划)(编号:2015CB856405)
国家科技支撑计划项目(编号:2015BAI13B01)
国家自然科学基金资助项目(编号:81571340)
关键词
注意缺陷多动障碍
睡眠
基因
反应抑制
Attention-deficit/hyperactivity
disorder
Sleep Gene
Reactive inhibition
