摘要
目的:观察高糖、高血管内皮生长因子(VEGF)及高IL-6下Müller细胞Kir4.1通道蛋白的变化。以及高糖、高VEGF及高IL-6环境下使用Kir4.1通道激活剂对kir4.1通道蛋白的影响。方法:(1)取SD大鼠幼鼠视网膜组织,进行视网膜Müller细胞的原代培养并进行Müller细胞特异性的鉴定。(2)对照组、高葡萄糖(30 mmol/L)、高VEGF(10 ng/m L)及IL-6(10 ng/m L)处理Müller细胞,2、4、16 h。Western BLot技术检测Kir4.1的蛋白含量变化。免疫荧光检测Kir4.1荧光含量变化。(3)高糖,高VEGF,高IL-6处理的Müller细胞,加入K^+通道开放剂吡那地尔和K^+通道抑制剂氯化钡,分别干预16 h。Western Blot技术检测Kir4.1的蛋白含量变化。结果:(1)高糖、高VEGF、IL-6干预组,Western Blot检测,干预16 h后均出现Kir4.1蛋白含量的明显下降。免疫荧光检测:高糖、高VEGF、IL-6干预16 h,Kir4.1蛋白荧光强度明显下降。(2)K^+通道干预剂干预16 h后,Western Blot检测Kir4.1蛋白含量结果 :高糖,高VEGF,高IL-6环境下,吡那地尔干预组,Kir4.1蛋白增多。结论:(1)高糖、高VEGF、高IL-6会引起Müller细胞上Kir4.1通道蛋白的含量下降。(2)在高糖环境,高VEGF,高IL-6环境下,使用K^+通道激动剂吡那地尔,会增加Müller细胞上Kir4.1的蛋白含量。
Objective To observe the changes of Kir4.1 channel protein with the use of high glucose,high vascular endothelial growth factor（VEGF）and IL-6 intervention, and the effects of Kir4.1 pathway protein by using Kir4.1 channel activator. Methods（1）SD-rat′ s retinal Müller cells were primary cultured and identified.（2）Changes in Kir4.1 protein content were detected by Western blot and immunofluorescence after intervention with high glucose（30 mmol / L）, VEGF（30 mmol / L） and IL-6（30 mmol / L） at 2, 4 and 16 h.（3）High glucose, VEGF and IL-6 treated Müller cells joined the K-＋channel activator pinacidil（10 μmol / L）and inhibitor barium chloride（10 μmol / L） of the K-＋channel for 16 h of interference. The changes in Kir4.1protein content were detected by Western blot. Results（1）In the high glucose group, the high VEGF group and the IL-6 intervention group, Müller cell Kir4.1 channel protein decreased at16 hours.（2）In the high glucose,high VEGF and high IL-6 environment, Kir4.1 protein of Müller cell increased with the use of the K-＋channel agonist, pinacidil. Conclusion High glucose, high VEGF and IL-6 intervention could make Kir4.1 channel protein of Müller cell decreased,while K-＋channel activator could make Kir4.1 channel protein increased.
出处
《实用医学杂志》
CAS
北大核心
2016年第9期1387-1391,共5页
The Journal of Practical Medicine
基金
国家自然科学基金(编号:81570876)
