摘要
以蒸馏水为溶剂回流提取水飞蓟种子,提取物依次过D101大孔树脂、MCI树脂、MPLC色谱、HPLC色谱等色谱分离纯化得到7个化合物。经NMR解析确定化合物1~7分别为tricin 4'-O-[threo-β-guaiacyl-(7″-O-methyl)-glyceryl]ether(1),tricin 4'-O-[erythro-β-guaiacyl-(7″-O-methyl)-glyceryl]ether(2),5'-methoxyhydnocarpin-D(3),palstatin(4),(8R,7'S,8'R)-5,5'-dimethoxy-7-oxolariciresinol 9'-O-D-xylopyranoside(5),9-O-D-glucopyranoside(6),(-)-haplomyrtoside(7)。化合物1,3~5具有较强的抗流感病毒(H5N1)活性,IC_(50)分别为0.65,0.21,0.32和0.56μmol·L^(-1),SI(SI=TC50/IC_(50))分别为12.5,3.5,10.6,7.8。化合物1,2,6,7具有较好的抗人肝癌细胞(Hep G-2)活性,IC_(50)分别为0.35,0.25,0.53,0.66μmol·L^(-1)。化合物1~7为从该植物中首次分离得到。
The seeds of Silybum marianum were extracted by hot water,and the extract was isolated by D101 macroporous resin,MCI resin,MPLC,HPLC,et al. As a result,7 compounds including tricin 4＇-O-[threo-β-guaiacyl-（ 7″-O-methyl）-glyceryl] ether（ 1）,tricin 4＇-O-[erythro-β-guaiacyl-（ 7″-O-methyl）-glyceryl] ether（ 2）,5＇-methoxyhydnocarpin-D（ 3）,palstatin（ 4）,（ 8R,7＇ S,8＇R）-5,5＇-dimethoxy-7-oxolariciresinol 9＇-O-D-xylopyranoside（ 5）,9-O-D-glucopyranoside（ 6）,and（-）-haplomyrtoside（ 7） were isolated and identified for the first time. Compounds 1,3,4,and 5 exhibited activity against influenza A（ H5N1） with IC50 value of 0. 65,0. 21,0. 32,and 0. 56 μmol·L-（-1）,respectively. Compounds 1,2,6,and 7 exhibited cytotoxity against Hep G-2 with IC_（50） value of0. 35,0. 25,0. 53,0. 66 μmol·L-（-1）,respectively.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2016年第9期1654-1657,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81274035)
关键词
水飞蓟
木脂素
抗流感病毒活性
细胞毒活性
Silybum marianum
lignan
anti-influenza virus activity
cytotoxity
