摘要
为了全面了解艾纳香中萜类物质的代谢途径,以及具体的基因和催化酶类,以期为从分子水平调控艾纳香中萜类活性成分提供理论依据。基于艾纳香的转录组数据,将艾纳香的转录组测序结果同KEGG数据库中其他多种高等植物萜类合成途径的研究结论进行比对,预测出艾纳香中萜类代谢的具体途径。结果表明艾纳香在KEGG数据库中比对上4条萜类代谢通路:萜类骨架代谢途径、单萜代谢途径、二萜代谢途径、倍半萜与三萜代谢途径,对应基因的数目分别为103,10,29,59条。通过对催化酶与活性产物进行总结分析,结果显示,单萜活性产物为8种、二萜3种、三萜和倍半萜3种。在萜类代谢途径过程中的关键酶主要为脱氧木酮糖-5-磷酸合酶、HMG-Co A还原酶、烯丙基转移酶系。艾纳香中与单萜类物质合成的酶类相对较少,而产物种类较多,这可能与单萜合酶催化产物的不专一性有关。通过对艾纳香中萜类调控途径的整体分析,并对其中关键酶基因的调控,有望整体提高艾纳香中萜类活性物质的产量。
In order to provide a theoretical basis for the regulation of active ingredient,the terpenoids metabolic pathway and specific enzymes in Blumea balsamifera are investigated. Basing on transcriptome information,B. balsamifera terpenoids metabolic pathway was analyzed in KEGG data base. Four metabolic pathway of terpenoids were found in KEGG data base. They were terpenoid backbone biosynthesis,monoterpenoid biosynthesis,diterpenoid biosynthesis,sesquiterpenoid and triterpenoid biosynthesis,contained 103,10,29,59 genes,respectively. Through the analysis of the enzyme and product in the pathway,the result showed that there were 8 kinds of monoterpenes,3 kinds of diterpenes,3 kinds of triterpenes and sesquiterpenes. The mainly key enzymes were deoxyxylulose 5-phosphate synthase,HMG-Co A reductase and allyl transferase system. In B. balsamifera,there were relatively few monoterpenes synthetic enzymes,while the type of products was much more than other terpenes. This may be relate to the non-specific catalytic characteristic of monoterpene synthase. It is expected to improve the yield of terpenoids in B. balsamifera by analysis the pathways and regulation the key enzymes.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2016年第9期1585-1591,共7页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81202910)
中央级公益性科研院所基本科研业务费专项资金项目(1630032014015)
海南省中药现代化专项(ZY201410)
关键词
艾纳香
转录组
萜类代谢途径
限速酶
Blumea balsamifera
transcription group
metabolic pathway of terpenoids
rate-limiting enzyme
