摘要
目的探讨氧体积分数对新生小鼠肺组织损伤程度的影响.方法新生1d的C57BL/6小鼠96只,随机分为:氧气Ⅰ组(FiO2:0.40)、氧气Ⅱ组(FiO2:0.60)、氧气Ⅲ组(FiO2:0.90)及空气对照组,每组24只.各组于24,48及72h随机取8只小鼠肺组织标本,测定肺湿/干重(W/D)比值,观察肺组织病理变化,进行病理评分及支气管肺泡灌洗液(BALF)中细胞总数、分数计数.结果随着氧体积分数的升高及给氧时间的延长,新生小鼠肺组织逐渐出现肺泡结构紊乱、间质细胞数量增多及肺水肿等损伤表现.48h氧气Ⅲ组、72h各氧气组肺组织W/D比值及病理评分较空气对照组更有统计学差异(p<0.05).与空气对照组比较,48h氧气Ⅱ、Ⅲ组及72h各氧气组BALF中细胞总数增多(p<0.05);各时间点各氧气组中性粒细胞、巨噬细胞计数显著升高(p<0.01).结论新生鼠吸入不同体积分数氧气48h均可引起典型的肺损伤,且随着氧体积分数的升高及给氧时间的延长肺损伤程度加重.
To observe oxygen concentration to the lungs of newborn mice with injury degree,totally 96 postnatal day 1mice,have been randomly divided into oxygen exposed group Ⅰ,oxygen exposed groupⅡ,oxygen exposed groupⅢ and air control group（n=24）respectively.The wet/dry weight（W/D）ratio has been assessed,the changes of lung pathology observed,and the lung injury score,total cells count and differential counting in bronchoalveolar lavage fluid（BALF）examined.With the time and concentration of exposure to oxygen,lung tissue gradually showed structure disruption,increased interstitial cell and pulmonary edema of lung injury.Compared with the air control group,lung W/D ratio and injury scores of 48 hours oxygen exposed group Ⅲ and all 72 hours oxygen exposed group（all p〈0.05）were noted increased.48 hours oxygen exposed groupⅡ,Ⅲand all 72 hours oxygen exposed group the total cells count in BALF were significantly higher than those air control group（p〈0.05）.The neutrophils and macrophages counting in all hours oxygen exposed group were noted increased compared with the air control group（p〈0.01）.It is concluded that exposure to differential oxygen concentration for 48 hours can induced the typical lung injury in newborn mice,the lung injury can became better worse with higher oxygen concentration and longer exposure times.
出处
《西南师范大学学报（自然科学版）》
CAS
北大核心
2016年第5期190-193,共4页
Journal of Southwest China Normal University(Natural Science Edition)
关键词
氧体积分数
新生小鼠
肺损伤
oxygen concentration
newborn mice
lung injury
