摘要
免疫磁珠分离技术在生物检测中的应用日益广泛,能提供高强度大梯度磁场环境的纳米磁珠分离器是免疫磁珠分离技术中的关键技术之一。设计了一种新颖的环形六孔纳米磁珠分离器,通过瓦状钕铁硼磁块的优化布局和斜壁坡莫合金导磁片设计,实现了在同一台纳米磁珠分离器上形成6个强磁场高梯度分离区域（分离孔）,分离器最高磁场强度达1.44 T,最高磁场梯度达96.3 T/m（体积865.5 cm3,质量4.8 kg）,同一分离器内6个分离孔内磁场分布相同,孔间平均相对偏差在2.0%~3.3%之间。对磁分离孔磁场分布的测试和仿真结果表明：磁珠分离器体积、导磁片材料和导磁片形状等因素对磁珠分离器磁场强度和梯度有不同程度的影响,采用大体积磁块的磁珠分离器比采用小体积磁块的磁珠分离器、采用坡莫合金作为导磁片比采用软铁材料作为导磁片、采用斜壁形状的导磁片比采用直壁形状的导磁片均能实现较大的磁场强度和梯度。该纳米磁珠分离器已成功应用于大肠杆菌和禽流感病毒的免疫磁分离试验研究,并可以根据不同应用需求,组合相关要素以获得理想的免疫磁珠分离器。
Immunomagnetic beads separation（ IMBS） technique plays more and more important roles in biological detection recently. One of the key technologies for immunomagnetic beads separation is the magnetic nanobead separator（ MNS） which is capable of providing a magnetic field of high intensity and high gradient. The innovative MNS was constructed by Nd Fe B magnetic tile blocks and permalloy clinohedral magnetizers. There are six separation holes on this annular MNS providing with the magnetic field of high intensity and high gradient. The MNS was successfully applied in the field of nanobead magnetic separation on both Escherichia coli and avian influenza virus. The magnetic intensity measurement results show that the highest MNS magnetic field intensity of 1. 44 T and the highest MNSmagnetic field gradient of 96. 3 T / m（ 865. 5 cm3 volume,4. 8 kg weight） were achieved. The measurement average relative error among each hole of the four sets of MNS was between 2. 0% to3. 3%. The study results also show that MNS volume,material and profile of magnetizer have different effects on the intensity and gradient of magnetic field. The larger MNS or the MNS with permalloy magnetizer or with sidleing-magnetizer can obtained higher intensity and gradient of the magnetic field compared to the smaller MNS or MNS with soft iron magnetizer or MNS with straight-shape magnetizer.Therefore,according to different IMBS application requirements,it can be obtained the ideal separator easily with different structure elements.
出处
《农业机械学报》
EI
CAS
CSCD
北大核心
2016年第5期315-320,335,共7页
Transactions of the Chinese Society for Agricultural Machinery
基金
科技部国家科技交流与合作专项(2010DFA31000)
关键词
免疫磁分离
磁珠分离器
磁场
分离效率
immunomagnetic beads separation
magnetic nanobead separator
magnetic field
separation efficiency
