摘要
目的了解产生梅毒血清学假阳性的生物学因素,从而提高对梅毒血清学生物假阳性的认识。方法选择该院各科室2013年5月至2014年10月收治的住院患者13 000例作为研究对象,研究对象均常规抽取新鲜空腹血样2mL,离心获得血清标本后,行梅毒螺旋体明胶凝集试验(TPPA),并在行快速血浆反应素环状卡片试验(RPR),RPR定性试验阳性者加检RPR血清滴度,检测结果应用SPSS22.0软件进行Logistic单因素、多因素回归分析。结果 13 000例血清标本中,RPR(+)、TPPA(+)者99例,RPR(-)、TPPA(+)者100例,RPR(+)、TPPA(-)者252例,假阳性率为1.94%(252/13 000)。单因素统计分析显示:风湿免疫系统疾病、年龄60岁以上老年患者、恶性肿瘤可能是梅毒血清学假阳性反应的影响因素,且差异有统计学意义(P<0.05);多因素Logistic回归分析发现风湿免疫系统疾病、感染性疾病和恶性肿瘤疾病可能是梅毒血清学假阳性反应的独立危险因素(P<0.05)。结论 RPR试验检测血清标本会出现生物假阳性的结果,必须用TPPA试验进行确认。
Objective To study the biological factors of Syphilis Serological false positive,and to improve the understanding of the false positive of syphilis serological biology knowledge.Methods In different departments of our hospital,from May 2013 to October 2014,13 000 patients were selected as the research subjects.2mL of fresh fasting blood were collected and centrifugalized.Serum specimens were given treponema pallidum agglutination test（TPPA）and rapid plasma reagin test（RPR）.The results of RPR qualitation test showing positive specimens were given RPR serum titer detection.All data results were tested by univariate and multivariate Logistic regression by SPSS22.0software.Results In 13 000 cases serum,RPR（＋）with TPPA（＋）were 99 cases,RPR（-）with TPPA（＋）were 100 cases,RPR（＋）with TPPA（-）were 252 cases respectively.The false positive rate was 1.94%（252/13 000）.Single factor statistical analysis showed that rheumatoid immune system disease,over 60 years old and malignant tumor were influence factors of false positive of syphilis serology detection（P〈0.05）.Multiple Logistic regression analysis showed that rheumatoid immune system disease,infection diseases and malignant diseases were the independent risk factors of false positive serological reactions（P〈0.05）.Conclusion The results of RPR test for the biological may be false positive in serum,which must be confirmed by TPPA test.
出处
《国际检验医学杂志》
CAS
2016年第10期1362-1364,1367,共4页
International Journal of Laboratory Medicine
